BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
氮素是构成生物体的必需元素之一，自然界中的氮素循环涉及多种转化过程：空气中的氮气被固氮微生物及植物-微生物共生体固定为氨态氮，再经硝化微生物作用转化为硝态氮，后者进一步被植物或微生物同化为有机氮化物。因此，植物组织中氨态氮含量可反映其受胁迫程度。本试剂盒的测定原理基于茚三酮显色反应：α-氨基酸与水合茚三酮共加热，经氧化脱氨生成相应α-酮酸，后者脱羧形成醛，同时水合茚三酮被还原；在弱酸环境中，还原型茚三酮、氨与另一分子水合茚三酮缩合生成蓝紫色物质，该产物在580 nm处具有特征吸收峰，通过测定吸光度即可定量分析氨基酸含量。
Nitrogen is one of the essential elements for living organisms. The nitrogen cycle in nature involves multiple transformation processes: atmospheric nitrogen (N₂) is fixed into ammonia‑nitrogen (NH₄⁺‑N) by nitrogen‑fixing microorganisms and plant‑microbe symbionts, which is then converted to nitrate‑nitrogen (NO₃⁻‑N) through the action of nitrifying microorganisms, and subsequently assimilated by plants or microorganisms into organic nitrogenous compounds. Therefore, the ammonia‑nitrogen content in plant tissues can reflect the degree of stress they are experiencing. The assay principle of this kit is based on the ninhydrin color reaction: α‑amino acids are heated with ninhydrin hydrate, undergoing oxidative deamination to yield the corresponding α‑keto acids, which are decarboxylated to form aldehydes, while ninhydrin hydrate is reduced. In a weakly acidic environment, the reduced ninhydrin, ammonia, and another molecule of ninhydrin hydrate condense to form a blue‑purple product that exhibits a characteristic absorption peak at 580 nm. By measuring the absorbance, the amino acid content can be quantitatively analyzed.
危险品化学品经营许可证（带存储）