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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果糖-1,6-二磷酸酶（FBP，EC 3.1.3.11）是糖异生和蔗糖合成中的关键酶。在光合细胞中，FBP存在两种亚型：胞质型FBP主要分布于细胞质，参与蔗糖合成与糖异生途径；叶绿体型FBP位于叶绿体，在CO₂同化途径中发挥调节作用。 FBP催化果糖-1,6-二磷酸水解为果糖-6-磷酸和无机磷，偶联后续酶促反应生成NADPH。通过监测340 nm处NADPH的生成速率，即可计算FBP的活性。
Fructose‑1,6‑bisphosphatase (FBP, EC 3.1.3.11) is a key enzyme in gluconeogenesis and sucrose synthesis. In photosynthetic cells, FBP exists in two isoforms: the cytosolic isoform, which is mainly distributed in the cytoplasm and participates in sucrose synthesis and gluconeogenesis; and the chloroplast isoform, which is located in the chloroplast and plays a regulatory role in the CO₂ assimilation pathway. FBP catalyzes the hydrolysis of fructose‑1,6‑bisphosphate to fructose‑6‑phosphate and inorganic phosphate (Pi). This reaction is coupled to downstream enzymatic reactions that generate NADPH. The FBP activity can be calculated by monitoring the rate of NADPH production at 340 nm.
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