计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用经典的DAB染色法检测植物组织中的活性氧。其核心原理为:过氧化氢(H₂O₂)在过氧化物酶催化下,将无色的DAB(3,3′-二氨基联苯胺)氧化为不溶于水的棕红色聚合产物(即DAB的氧化形式),并原位稳定沉积于反应位点。通过分析该棕色沉淀的分布范围与着色深度,即可实现H₂O₂积累位点的直接定位与半定量分析,从而表征氧化应激发生的具体部位与相对强度。
This kit employs the classic DAB staining method for the detection of reactive oxygen species (ROS) in plant tissues. The core principle is as follows: In the presence of peroxidase, hydrogen peroxide (H₂O₂) oxidizes colorless DAB (3,3′‑diaminobenzidine) to an insoluble brown‑red polymeric product (the oxidized form of DAB), which is stably deposited in situ at the reaction site. By analyzing the distribution range and staining intensity of the brown precipitate, the accumulation sites of H₂O₂ can be directly localized and semi‑quantitatively analyzed, thereby characterizing the specific locations and relative intensity of oxidative stress occurrence.