I. Formula (per liter)
Casein 5.0 g
Anhydrous glucose 40.0 g
L-cystine 0.4 g
Sodium acetate trihydrate 20.0 g
Riboflavin 0.4 mg
L-tryptophan 0.1 g
Thiamine hydrochloride 0.2 mg
Adenine sulfate 20.0 mg
Guanine hydrochloride 20.0 mg
Uracil 20.0 mg
Magnesium sulfate heptahydrate 0.4 g
Biotin 0.8 µg
Nicotinic acid (niacin) 1.0 mg
p-Aminobenzoic acid 0.2 mg
Pyridoxine hydrochloride 0.8 mg
Sodium chloride 20.0 mg
Polysorbate 80 (Tween 80) 0.1 g
Manganese sulfate monohydrate 20.0 mg
Ferrous sulfate heptahydrate 20.0 mg
Potassium dihydrogen phosphate trihydrate 1.0 g
Dipotassium hydrogen phosphate 1.0 g
Final pH: 6.8 ± 0.1 (25°C)
II. Preparation Method (100 mL)
Weigh 6.81 g of this product in 100 mL of distilled or deionized water (the volume may be scaled up or down proportionally), adjust to a final pH of 6.8 ± 0.1 (25°C), heat with stirring and boil for 2 minutes until completely dissolved, dispense (stir well to ensure even distribution of insoluble matter), and set aside for use.
III. Precautions
1. Avoid contact with skin and eyes during use.
2. This product is for laboratory microbiological testing.
危险品化学品经营许可证（带存储）