BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
植物组织培养技术即植物无菌培养技术，又称离体培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官 (如根、茎、叶、花、茎尖、果实等)、组织 (如形成层、表皮、表层、髓部细胞、胚乳等) 或细胞 (大孢子、小孢子、体细胞等) 以及原生质体，在适宜的人工培养基及环境条件下，能够诱导出愈伤组织、不定芽、不定根、最后形成完整植株的技术。在配制培养基前，为了使用方便、简化操作、用量准确，减少每次配药称量各种化学成分所花费的时间和误差，常将配制培养基所需无机大量元素、微量元素、铁盐、有机物、激素成分分别配制成比需要量大若干倍的浓缩母液，当配制培养基时按预先计算好的量分别吸取各种母液即可。
植物营养液‑微量元素母液 (400×) 主要由碘化钾、硼酸、硫酸锰、硫酸锌、硫酸铜、钼酸钠等组成，可用于配制霍格兰 (Hoagland's) 营养液等常用的植物营养液，用于培养植物组织，检测植物生长速率。使用时稀释至 1×，并加入其他组分，每 100mL 溶液可制备 40L 营养液。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、蒸馏水或去离子水、稀酸、稀碱、大量元素母液、铁盐母液、有机物、激素。
2、pH 计、磁力搅拌器、烧杯。
操作步骤 (仅供参考)：
1、取一升烧杯，加入 800mL 去离子水，加入 2.5mL 微量元素母液 (400×)，搅拌状态下再依次加入大量元素母液、铁盐母液、有机物、激素等成分，再用稀酸或稀碱调节 pH 至 5.5‑5.9，补水至一升，即为相关的营养液。配制完成后的营养液，4℃ 条件下保存 1 个月有效。
2、如需做半剂量 (1/2 剂量)，则本试剂用量减半。
注意事项：
1、要根据不同的材料、不同的物种，选择合适的培养基，最好通过实验获得。
2、营养液 pH 值以 5.0‑6.5 为宜。如需严格无菌，可 121℃高压灭菌 15‑20 min。
3、 试剂开封后请尽快使用，以免影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Plant tissue culture technology, also known as plant aseptic culture technology or in‑vitro culture technology, is based on the theory of plant cell totipotency. Under suitable artificial culture medium and environmental conditions, the isolated plant organs (such as roots, stems, leaves, flowers, shoot‑tips, fruits, etc.), tissues (such as cambium, epidermis, surface layer, pith cells, endosperm, etc.), cells (megaspores, microspores, somatic cells, etc.) and protoplasts can be induced to produce callus, adventitious buds and adventitious roots, and finally develop into intact plants. Prior to medium preparation, to facilitate operation, simplify procedures, ensure accurate dosage and reduce the time‑cost and weighing error of chemical components, inorganic macro‑elements, micro‑elements, iron salts, organic substances and hormones required for culture media are usually prepared separately as concentrated stock solutions with several‑fold higher concentrations. Corresponding volumes of each stock solution can be pipetted as pre‑calculated during medium preparation.
Plant Nutrient Solution‑Trace Element Stock Solution (400×) is mainly composed of potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, sodium molybdate, etc. It can be used to prepare common plant nutrient solutions such as Hoagland’s solution for plant tissue culture and plant growth‑rate detection. Dilute to 1× before use and supplement with other components. 100 mL of this stock solution can be used to prepare 40 L of nutrient solution. This reagent is intended for research‑only applications and is not suitable for clinical diagnosis or other purposes.
Materials to Be Prepared:
1. Distilled water or deionized water, dilute acid, dilute alkali, macro‑element stock solution, iron‑salt stock solution, organic substances, hormones.
2. pH meter, magnetic stirrer, beaker.
Operating Procedure (For Reference Only):
1. Take a 1‑L beaker, add 800 mL deionized water, then pipette 2.5 mL Micronutrient Stock Solution (400×). While stirring, add macro‑element stock solution, iron‑salt stock solution, organic substances, hormones and other components sequentially. Adjust the pH to 5.5‑5.9 with dilute acid or dilute alkali, then top‑up the volume to 1 L to obtain the target nutrient solution. The prepared nutrient solution is valid for 1 month when stored at 4 ℃.
2. If half‑strength (1/2 dosage) is required, halve the dosage of this reagent.
Precautions:
1. Select a suitable culture medium according to different materials and plant species. Optimal conditions are preferably determined experimentally.
2. The recommended pH range of nutrient solution is 5.0‑6.5. For strict sterility, perform autoclaving at 121 ℃ for 15‑20 min.
3. Use up the reagent promptly after opening to avoid adverse effects on subsequent experiments.
4. For your personal safety and health, please wear a lab coat and disposable gloves during operation.
危险品化学品经营许可证（带存储）