本品选用非疫区健康胎牛血液为原料，在GMP受控洁净环境下完成无菌采血、低温自然分离粗血清，采用专用温和去脂精制工艺，定向去除血清内甘油三酯、胆固醇、游离脂肪酸、脂溶性杂质及乳糜微粒，最大程度保留水溶性蛋白、生长因子与多肽活性组分，再经多级澄清、梯度过滤、0.1μm终端除菌过滤制备成品。
本品为低脂、无乳糜干扰专用血清，专门解决普通血清高脂背景导致的实验干扰问题，适配脂代谢研究、脂肪酸干预、脂质组学、动脉粥样硬化模型、脂肪细胞功能实验、脂溶性药物筛选等体系。工艺温和、无有机溶剂残留，不破坏核心水溶性活性营养，兼顾细胞正常生长与低脂实验本底要求，血源全程可追溯。
产品核心特点
1. 超低脂类本底：定向去除甘油三酯、总胆固醇、游离脂肪酸、乳糜颗粒， 总胆固醇＜10mg/dL ，大幅降低内源脂质干扰。
2. 活性保留度高：区别于广谱活性炭吸附，仅靶向去除脂类杂质，完整保留水溶性生长因子、蛋白与细胞营养。
3. 液体澄清度高：无乳糜浑浊，透光性好，适配吸光度检测、显色实验、微量样本分析。
4. 适配脂代谢精准实验：外源脂肪酸、脂质药物干预响应真实，数据重复性强。
5. 批间脂类指标高度统一，适合长期代谢机制研究、脂质组学测序。
适用范围
1. 专属适用：细胞脂质代谢研究、脂肪酸/胆固醇干预模型、动脉粥样硬化细胞模型、脂肪细胞分化培养、脂质组学、脂类药物筛选、脂蛋白功能实验、需要消除血清内源脂质干扰的科研体系。
2. 通用适用：常规肿瘤细胞、贴壁细胞系传代培养。
使用方法和储存
1. 解冻方式：-20℃以下取出，2～8℃缓慢解冻，轻柔颠倒混匀，禁止37℃快速直融，保护水溶性活性蛋白稳定。
2. 配比：常规细胞培养添加5%～10%；脂代谢造模、药物干预实验建议固定10%统一浓度，保证实验平行性。
3. 分装保存：大瓶解冻后无菌分装单次用量，-10℃以下冻存，规避反复冻融造成组分波动。
4. 热灭活：无特殊免疫需求不建议热灭活，避免蛋白变性影响细胞状态与实验背景。
5. 长期储存：-10℃及以下避光冷冻保存，严禁反复冻融。有效期可达5年。
6. 短期储存：完全解冻后2～8℃可保存7天，建议尽快配液使用。
注：去脂血清清亮度远优于普通血清，若出现轻微蛋白絮状物为正常现象，不影响低脂实验体系。
注意事项
1. 本品为低脂专用科研试剂，主打去除脂类、乳糜微粒，与活性炭、去激素、透析、去蛋白工艺血清用途完全不同，不可相互替代。
2. 脂代谢、组学实验对批次敏感度高，关键实验建议锁定同一批次血清。
3. 本品脂类含量极低，不可用于需要外源脂质补充的细胞培养体系。
4. 解冻后少量细微絮状物为正常现象；出现浑浊、变色、大量沉淀禁止使用。
5. 废弃试剂与耗材按照生物安全废弃物规范处置。
6. 本品仅用于体外科学研究、细胞培养实验，严禁用于人体临床、体内给药、药物成品生产及食品加工。
免责声明
本产品仅用于科研或工业用途，不适用于临床治疗。用户需根据自身实验体系验证血清适用性，生产方不承担因操作不当导致的实验结果偏差责任。
This product is manufactured using blood collected from healthy fetal bovines in non-epidemic areas as raw material. Under GMP-controlled clean environments, aseptic blood collection and low-temperature natural separation of crude serum are performed. A proprietary mild delipidation refining process is applied to selectively remove triglycerides, cholesterol, free fatty acids, lipid-soluble impurities and chylomicrons from serum, while maximally preserving water-soluble proteins, growth factors and bioactive peptide components. The finished product is obtained via multi-stage clarification, gradient filtration and 0.1 μm terminal sterile filtration.
This is a low-fat serum free of chylous interference, specially developed to resolve experimental artifacts caused by the high lipid background of conventional serum. It is compatible with lipid metabolism research, fatty acid intervention, lipidomics, atherosclerosis models, adipocyte function assays, lipophilic drug screening and other systems. The process is mild without organic solvent residues, does not degrade core water-soluble bioactive nutrients, meets both the requirements for normal cell growth and low lipid experimental background, and features fully traceable blood sources.
Key Product Features
1. Ultra-low lipid background: Selective removal of triglycerides, total cholesterol, free fatty acids and chylomicrons; total cholesterol <10 mg/dL, which greatly reduces endogenous lipid interference.
2. High retention of bioactivity: Different from broad-spectrum activated carbon adsorption, this process only targets lipid impurities and fully retains water-soluble growth factors, proteins and cellular nutrients.
3. High liquid clarity: Free of chylous turbidity with good light transmittance, suitable for absorbance measurement, colorimetric assays and micro-sample analysis.
4. Compatible with precise lipid metabolism experiments: Reliable responses to exogenous fatty acid and lipid drug interventions with excellent data reproducibility.
5. Highly consistent lipid indicators across batches, suitable for long-term metabolic mechanism studies and lipidomics sequencing.
Scope of Application
1. Exclusive applications: Cellular lipid metabolism research, fatty acid/cholesterol intervention models, atherosclerotic cell models, adipocyte differentiation culture, lipidomics, lipid drug screening, lipoprotein function assays, and other research systems requiring elimination of endogenous serum lipid interference.
2. General applications: Subculture of routine tumor cells and adherent cell lines.
Instructions for Use and Storage
1. Thawing: Remove from storage at ≤ -20 °C and thaw slowly at 2–8 °C. Mix gently by inverting. Rapid direct thawing at 37 °C is prohibited to stabilize water-soluble bioactive proteins.
2. Working concentration: Add 5%–10% for routine cell culture. A fixed concentration of 10% is recommended for lipid metabolism modeling and drug intervention experiments to ensure experimental parallelism.
3. Aliquot storage: Aseptically aliquot single-use portions after thawing the bulk bottle, and store frozen at ≤ -10 °C to avoid component fluctuations caused by repeated freeze-thaw cycles.
4. Heat inactivation: Heat inactivation is not recommended unless specific immunological requirements exist, to prevent protein denaturation that may compromise cell status and experimental background.
5. Long-term storage: Store frozen, protected from light at ≤ -10 °C. Repeated freeze-thaw cycles are strictly prohibited. Valid shelf life up to 5 years.
6. Short-term storage: After complete thawing, store at 2–8 °C for up to 7 days. Prepare medium and use promptly.
Note: Delipidated serum exhibits far superior clarity compared with conventional serum. Minor protein floccules are normal and will not affect low-lipid experimental systems.
Precautions
1. This is a low-lipid research-grade serum specially designed for removal of lipids and chylomicrons. It has a completely different intended use from activated carbon treated, hormone-depleted, dialyzed or protein-depleted serum and cannot be substituted interchangeably.
2. Lipid metabolism and omics experiments are highly batch-sensitive. For critical experiments, use serum from a single batch.
3. This product contains extremely low lipid levels and must NOT be used for cell culture systems requiring exogenous lipid supplementation.
4. Small fine floccules after thawing are normal. Do not use if turbidity, discoloration or massive precipitate occurs.
5. Discard waste reagents and consumables in compliance with biosafety waste regulations.
6. For in vitro scientific research and cell culture experiments ONLY. Prohibited for human clinical use, in vivo administration, finished pharmaceutical manufacturing or food processing.
Disclaimer
This product is intended for research or industrial purposes only and is not suitable for clinical therapy. Users shall verify the suitability of the serum for their own experimental systems. The manufacturer assumes no liability for deviations in experimental results arising from improper operation.
危险品化学品经营许可证（带存储）