计算溶液所需的质量、体积或浓度。
植物花粉管染色液(苯胺蓝-番红O染色法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
有花植物的花粉需要传送精细胞到达胚珠来完成双受精，在这个过程中花粉要克服两方面的障碍：一是花粉从花药中释放后到达雌性生殖器官——雌蕊，这个过程双性花依靠花药的开裂，单性花要在风或者昆虫的帮助下完成；二是花粉落到雌蕊的柱头上后，花粉要传送精细胞到卵细胞中，这是一个富有挑战的过程，因为卵细胞远离柱头，并且是封闭在雌配子体内部的胚珠当中。为了完成这样一个任务，花粉会长出一个花粉管来传送精细胞。花粉管的生长过程主要分为：花粉和柱头的粘附、花粉的水合、花粉管的萌发和生长、花粉管受珠孔的引导以及花粉管分化释放精细胞等过程。
植物花粉管染色体液（苯胺蓝-番红O染色法）由花粉管染色体液和透明液组成，雌蕊经水溶性苯胺蓝和番红O染色后，花柱组织染成极浅的紫色，而花粉管染成深蓝色，容易区分。本产品操作简单快捷，可利用普通光学显微镜对水稻、拟南芥等植物花粉管的生长过程进行观察。
该产品仅用于科研领域，不用于临床诊断或其他用途。
组分与存储
| 组分货号 | 组分名称 | 物理外观 | 2×50mL | 储存 |
| P1529788A | 花粉管染色液 | 液体 | 50mL | 室温 |
| P1529788B | 透明液 | 液体 | 50mL | 室温 |
自备材料：
1、新鲜的植物组织切片（花粉管）
2、AAF固定液（50%）或Carnoy固定液或AF固定液、梯度乙醇、乳酸酚或乳酚油
操作步骤：（仅供参考）
1、授粉后的雌蕊在酒精-三氯甲烷-醋酸（3:4:1）溶液中固定30 min。
2、在70%酒精、50%酒精、30%酒精、蒸馏水中逐一浸泡2 min后取出。
3、浸入花粉管染色体液染色30～120 min。
4、在透明液中透明60 min。
5、乳酸酚封片，普通光学显微镜下观察染色效果。
染色结果：
花粉管呈深蓝色，花柱呈极浅的紫色。
注意事项：
1、试剂开封后请尽快使用，以防影响后续实验效果。
2、本产品含有乙酸，有一定的挥发性和刺激性，使用后请及时拧紧瓶盖。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
In flowering plants, pollen needs to deliver sperm cells to the ovule to complete double fertilization. During this process, pollen must overcome two obstacles: first, after being released from the anther, the pollen must reach the female reproductive organ—the pistil. In bisexual flowers, this process relies on anther dehiscence, while in unisexual flowers, it is completed with the help of wind or insects. Second, after the pollen lands on the stigma of the pistil, it must deliver sperm cells to the egg cell. This is a challenging process because the egg cell is far from the stigma and is enclosed within the ovule inside the female gametophyte. To accomplish this task, the pollen grows a pollen tube to deliver the sperm cells. The growth process of the pollen tube mainly includes: adhesion between pollen and stigma, pollen hydration, pollen tube germination and growth, guidance of the pollen tube by the micropyle, and differentiation of the pollen tube to release sperm cells.
Plant Pollen Tube Chromosome Staining Solution (Aniline Blue-Safranin O Staining Method) consists of Pollen Tube Chromosome Staining Solution and Clearing Solution. After the pistil is stained with water-soluble aniline blue and Safranin O, the style tissue is stained a very light purple, while the pollen tube is stained dark blue, making them easy to distinguish. This product is simple and rapid to use, and the growth process of pollen tubes in plants such as rice and Arabidopsis can be observed using an ordinary optical microscope.
This product is for research use only and is not intended for clinical diagnosis or other purposes.
Contents & Storage
| Component No. | Component Name | Appearance | 2×50mL | Storage |
| P1529788A | Plant Pollen Tube Staining Solution | Liquid | 50mL | Room Temperature |
| P1529788B | Clearing Solution | Liquid | 50mL | Room Temperature |
Materials Required but Not Provided:
1.Fresh plant tissue sections (pollen tubes)
2.AAF fixative (50%) or Carnoy's fixative or AF fixative, gradient ethanol, lactophenol or lactophenol oil
Procedure: (for reference only)
1.Fix the pollinated pistil in alcohol-chloroform-acetic acid (3:4:1) solution for 30 min.
2.Soak sequentially in 70% alcohol, 50% alcohol, 30% alcohol, and distilled water for 2 min each, then remove.
3.Immerse in Pollen Tube Chromosome Staining Solution and stain for 30–120 min.
4.Clear in Clearing Solution for 60 min.
5.Mount with lactophenol and observe the staining effect under an ordinary optical microscope.
Staining Results:
The pollen tube appears dark blue, and the style appears very light purple.
Precautions:
1.After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
2.This product contains acetic acid, which is somewhat volatile and irritant. Please tighten the cap promptly after use.
3.For your safety and health, please wear a laboratory coat and disposable gloves during operation.
规格
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
Store at Room temperature long term (12 months).
英文名称
Plant Pollen Tube Staining Solution (Aniline Blue‑Safranin O Staining Method)
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
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