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PVLG溶液
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为什么选择此级别
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
PVLG溶液又称PVLG染色液，由乳酸、聚乙烯醇、甘油等组成。用于真菌鉴定染色及检测，如丛植菌根的染色等。该试剂常用作封固剂或浮载剂，常与梅尔泽试剂 (阿拉丁货号：M1508164)一起使用。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、待测样品、梅尔泽试剂、氢氧化钾溶液
2、载玻片、盖玻片、显微镜、解剖针或移液管、镊子
操作步骤（仅供参考）：
1、在载玻片的一端加两滴PVLG溶液，另一端加一滴PVLG溶液和一滴梅尔泽试剂。
2、在显微镜下挑出形态相似的孢子，用解剖针或移液管把10个左右孢子小心的放在两端试剂中，使用移液管时要尽量减少落在试剂中的水份。
3、静置染色5 min，在显微镜下，用铅笔、镊子或是解剖针钝的一端直接按压在盖玻片上。
4、镜检。观察孢子的特异反应；对有代表性或特异性的特征随时拍照。
染色结果：
1、淀粉质或梅尔泽阳性反应：被测组织颜色呈蓝色到黑色
2、拟淀粉质或似糊精反应：被测组织呈棕色到红棕色
3、非淀粉质或梅尔泽阴性反应：被测组织不改变颜色，或显浅黄棕色
4、真淀粉质反应：被测组织可在未经氢氧化钾预处理的情况下变蓝
5、半淀粉质反应：被测组织在鲁戈尔溶液中变红，但在梅尔泽试剂中没任何反应；用氢氧化钾预处理后在两种试剂中均变蓝
注意事项：
1、大部分孢子在梅尔泽试剂和鲁戈尔试剂中产生不同的反应结果。有些孢子在梅尔泽试剂中无反应，但在鲁戈尔试剂中，结果从微弱或无反应，到碘阳性反应；有些孢子在梅尔泽试剂中给出淀粉质反应而在鲁戈尔试剂中甚至给出拟淀粉质反应。
2、当与碱性溶液混合时，梅尔泽试剂退化为浑浊的沉淀，因此它不能跟像氢氧化钾或氨水这样的常用真菌学试剂混合使用或直接相继使用。当用氢氧化钾做预处理时，必须在加梅尔泽试剂前中和碱性。
3、当梅尔泽试剂与PVLG溶液1:1混合使用时，染色反应会略微减小，但是不足以影响整个反应的颜色变化。装片不能保持很久，染色反应通常会在1-2年之内褪去。
4、静置5 min后压片的目的是使试剂略微变干，增加边缘的粘性，压片的时候减少液体流动导致孢子滑动到盖玻片以外。
5、在显微镜下，用铅笔、镊子或是解剖针钝的一端直接按压在盖玻片上，相当于间接对个别孢子施压，这样每个孢子被按压的程度不同，这样就会显露出内部存在的原本很难看见的细微有弹性的内壁。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
染色效果：
 淀粉质反应：白乳菇孢子，100 倍 |  拟淀粉质反应：高大环柄菇，100 倍 |
PVLG Solution，also known as PVLG staining solution, consists of lactic acid, polyvinyl alcohol, glycerol and other components. It is used for staining and detection in fungal identification, such as the staining of arbuscular mycorrhizae. This reagent is commonly used as a mounting medium or floating medium and is often applied together with Melzer’s Reagent (Aladdin Cat. No.: M1508164).
For research‑only use. Not intended for clinical diagnosis or other applications.
Materials to be prepared by user:
1. Test samples, Melzer’s reagent, potassium hydroxide solution
2. Slides, coverslips, microscope, dissecting needle or pipette, forceps
Procedure (for reference only):
1. Add two drops of PVLG solution to one end of the slide, and one drop of PVLG solution plus one drop of Melzer’s reagent to the other end.
2. Pick spores of similar morphology under the microscope. Carefully place approximately 10 spores into the two reagent droplets with a dissecting needle or pipette. Minimize extra water introduced into the reagents when using a pipette.
3. Allow staining for 5 min. Under the microscope, press the coverslip gently with a pencil, forceps or the blunt end of a dissecting needle.
4. Microscopic observation. Examine the specific reaction of spores; take photographs for representative or distinctive features at any time.
Staining Results:
1. Amyloid or Melzer’s positive reaction: The tested tissue appears blue to black.
2. Pseudoamyloid or dextrinoid reaction: The tested tissue appears brown to reddish‑brown.
3. Non‑amyloid or Melzer’s negative reaction: The tested tissue shows no colour change, or appears pale yellowish‑brown.
4. Euthyloid reaction: The tested tissue turns blue without potassium hydroxide pre‑treatment.
5. Hemiamyloid reaction: The tested tissue turns red in Lugol’s solution but shows no reaction in Melzer’s reagent; after potassium hydroxide pre‑treatment, it turns blue in both reagents.
Precautions:
1. Most spores yield different reaction results in Melzer’s reagent and Lugol’s solution. Some spores show no reaction in Melzer’s reagent, while reactions in Lugol’s solution range from faint or no response to iodine‑positive reactions. Some spores exhibit amyloid reaction in Melzer’s reagent but pseudoamyloid reaction in Lugol’s solution.
2. When mixed with alkaline solutions, Melzer’s reagent degrades into turbid precipitates. Therefore, it cannot be mixed or applied sequentially with common mycological reagents such as potassium hydroxide or ammonia water. If pre‑treated with potassium hydroxide, the alkalinity must be neutralized before adding Melzer’s reagent.
3. When Melzer’s reagent is mixed with PVLG solution at a ratio of 1:1, the staining response decreases slightly, yet the colour change is still distinguishable. The mounts are not permanent; staining reactions usually fade within 1–2 years.
4. The purpose of pressing the coverslip after 5 min of incubation is to let the reagent slightly dry, increase edge viscosity and reduce spore sliding out from under the coverslip caused by liquid flow during pressing.
5. Pressing the coverslip under the microscope with a pencil, forceps or the blunt end of a dissecting needle applies indirect pressure to individual spores. Spores receive uneven compression, which helps reveal delicate, elastic inner walls that are normally difficult to observe.
6. For personal safety and health, wear a lab coat and disposable gloves during operation.
7. Use the reagent promptly after opening to avoid compromised experimental results.
Staining Results:
Amyloid reaction: Spores of Lactarius piperatus, 100× | Dextrinoid reaction: Macrolepiota procera, 100× |
规格
别名
PVLG 封固液 | PVLG 染色液 | 丛枝菌根封固液 | PVLG染色液
英文别名
Polyvinyllactoglycerol Mountant
规格或纯度
BioReagent, 生物染色剂, 适用于微生物学, 用于显微镜
稳定性与储存
Store at RT long term (12 months).
英文名称
PVLG Solution
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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