BioReagent,Suitable for Flow Cytometry,即用型,无叠氮钠,适用于免疫荧光(IF) 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
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文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
物理外观： Liquid
储存缓冲液：PBS pH 7.4, containing 1% BSA and 0.05% Proclin300
Universal Antibody Diluent Buffer is a ready to use PBS based buffer containing Proclin300 and Bovine Serum Albumin (BSA) that has been developed for stability during long-term storage and for improved antibody performance. This product can be used for the dilution and formulation of primary or secondary antibodies, including biotinylated antibodies and fluorescent labeled antibodies. This product has been tested to work in WB, IF/ICC, ELISA and Flow Cytometry. Instructions for Use： Perform appropriate fold dilutions of primary or secondary antibodies directly with Universal Antibody Diluent Buffer according to the antibody instructions for use. Antibodies preserved in Universal Antibody Diluent Buffer are recoverable for storage at 4℃ and can be used repeatedly multiple times until ideal results are not achieved then discarded.
BioReagent, Suitable for Flow Cytometry, 适用于免疫荧光(IF), 即用型, 无叠氮钠
稳定性与储存
The solution should be stored undiluted between 2°C and 8°C for 12 months. Do not freeze.
英文名称
Universal Antibody Diluent Buffer
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
图片
Universal Antibody Diluent Buffer (P187048) - IF/ICC IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). The biotinylated antibody and FITC labeled antibody were preserved in Universal Antibody Diluent Buffer (P187048). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Universal Antibody Diluent Buffer (P187048) - Flow Cytometry Flow cytometry analysis of CD3 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (PE) (Ab214237) at 5μL/test for 1 hour at 4°C. The PE labeled antibody was preserved in Universal Antibody Diluent Buffer (P187048). Left panel - Mouse IgG (PE) (Ab180308).
ZJ26F1039653
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