计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Features
Ultrapure quality
Agglutinates leucocytes and erythrocytes
Mixture of L and E subunits
Sugar specificity: complex oligosaccharides
Lyophilized powder
Product description
Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containeningL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.
Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.
Medicago’s PHA-P lectin is supplied as a white to lightpinkessentially salt-free lyophilized powder from 50 mMNH4HCO3, which evaporates during the lyophilizationprocedure. The purity of the lectin is determined by SDSPAGEgenerating one single band.
Applications
Lateral flow erythrocyte separation
Typical subunit composition
Total L subunit composition: 35-40%
Total E subunit composition: 60-65%
L4 0-10%
L3E1 10-20%
L2E2 15-25%
L1E3 35-50%
E4 10-20%
Directions for use
The lectin may be reconstituted in a suitable buffer for yourapplication.
Shipping and storage
The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.
H317: 可能引起皮肤过敏反应
H319: 引起严重眼睛刺激
H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。