计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Features
Ultrapure quality
Agglutinates leucocytes and erythrocytes
Mixture of L and E subunits
Sugar specificity: complex oligosaccharides
Lyophilized powder
Product description
Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.
Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.
PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band.
Applications
Lateral flow erythrocyte separation
Typical subunit composition
Total L subunit composition: 60-65%
Total E subunit composition: 35-40%
L4 25-35%
L3E1 20-30%
L2E2 10-20%
L1E3 17-27%
E4 1-11%
Directions for use
The lectin may be reconstituted in a suitable buffer for yourapplication.
Shipping and storage
The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.
H317: 可能引起皮肤过敏反应
H319: 引起严重眼睛刺激
H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。