A2404580计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 2 篇同行评审文献引用。
概述
聚醚 F127 二丙烯酸酯（
P477925
）为双键改性聚乙二醇-聚丙二醇-聚乙二醇三嵌段共
聚物，其可通过紫外及可见光在光引发剂作用下快速交联固化成胶。F127 具有优异的热致
凝胶（升温凝胶）特性和良好的生物安全性，基于 F127 的材料体系可被应用于生物医学领
域，如用作药物载体、伤口敷料、细胞载体剪切保护剂、生物 3D 打印等。
应用：
聚乙二醇-嵌段-聚丙二醇-嵌段-聚乙二醇二丙烯酸酯(PEG-PPG-PEG)是基于双官能团丙烯酸酯的两亲性三嵌段共聚物，可用于制药、生物医药和个人护理产品。它还可用于合成药物递送的交替嵌段聚氨酯。
储存及运输：
干态套装：室温，3 个月；4℃，12 个月；-20℃，18 个月。 无菌溶液：4°C 避光，7天；-20℃避光，6 个月。溶液反复冻融会影响产品性能，尽量现配现用。
溶液配制：
1.配制 0.25% (w/v) 引发剂标准溶液
(1) 取 20mL PBS，加入装有引发剂 LAP 的棕色瓶中(内含 0.05g LAP)；
(2) 以 40~50℃水浴加热溶解 15 分钟，期间手动或涡旋振荡 2~3 次，每次 5~10 秒。
该 LAP 标准液在 4°C 避光条件下可保存 12 个月。
2.配制
P477925
溶液（建议
P477925
浓度为 5~30 % w/v，5%以下适宜与其他水凝胶复合用于细胞 3D 培养）
(1) 取所需质量的
P477925
放入离心管。
(2) 取引发剂标准溶液加入到上述离心管中。
(3) 2~8℃溶解 30 分钟，期间手动或涡旋振荡 2~3 次，每次 5~10 秒（浓度＜20%可室温搅拌溶解）。
固化使用：
用 405nm 光源照射上述含引发剂的 P477925
溶液，10-30s 便可固化成胶，为获得更高强度水凝胶，可酌情延长光照时间。
说明：
P477925
溶液具有热致凝胶特性（升温凝胶），在配制溶液过程中，降温有利于溶解。建议溶液在 2-8℃静置溶解，期间用漩涡混匀器振荡数次。
浓度≥20%的
P477925
溶液，其在室温静置会出现凝胶化现象，浓度越高，越易凝胶化，此时的凝胶为物理可逆凝胶，温度降低到 2~8℃后会重新恢复溶液状态。
温馨提示：请勿直视固化光源。
半成品套件：
组分A：F127DA，白色粉末状，1g/瓶 or 5g/瓶：避光保存
组分B：光引发剂 LAP，白色粉末状，0.05g/瓶 * (1 or 5)：避光保存
Polyether F127 diacrylate (P477925) is a double bond modified polyethylene glycol polypropylene glycol polyethylene glycol triblock copolymer, which can be quickly crosslinked and cured into a gel by UV and visible light under the action of photoinitiators. F127 has excellent thermotropic gel (heating gel) characteristics and good biosafety. The material system based on F127 can be used in the biomedical field, such as as drug carrier, wound dressing, cell carrier shear protector, biological 3D printing, etc.
Application:
Polyethylene glycol block polypropylene glycol block polyethylene glycol diacrylate (PEG-PPG-PEG) is an amphiphilic triblock copolymer based on bifunctional acrylate, which can be used in pharmaceuticals, biopharmaceuticals, and personal care products. It can also be used for synthesizing alternating block polyurethanes for drug delivery.
Storage and transportation:
Dry set: room temperature, 3 months; 4 ℃, 12 months- 20 ℃, 18 months. Sterile solution: protected from light at 4 ° C for 7 days- Avoid light at 20 ℃ for 6 months. Repeated freezing and thawing of the solution can affect product performance, so it is recommended to prepare and use it as soon as possible.
Solution preparation:
1. Prepare a 0.25% (w/v) initiator standard solution
(1) Take 20mL PBS and add it to a brown bottle containing initiator LAP (containing 0.05g of LAP);
(2) Heat and dissolve in a 40-50 ℃ water bath for 15 minutes, during which manually or vortex oscillate 2-3 times for 5-10 seconds each time.
This LAP standard solution can be stored for 12 months under dark conditions at 4 ° C.
2. Prepare P477925 solution (the recommended concentration of P477925 is 5~30% w/v, and below 5% is suitable for combination with other hydrogels for 3D cell culture)
(1) Take the required mass of P477925 and place it in a centrifuge tube.
(2) Take the standard solution of the initiator and add it to the centrifuge tube mentioned above.
(3) Dissolve at 2-8 ℃ for 30 minutes, with 2-3 manual or vortex oscillations for 5-10 seconds each time (concentration<20% can be dissolved by stirring at room temperature).
Curing use:
Irradiate the above P477925 solution containing initiator with 405nm light source, and it can be cured into gel within 10-30s. In order to obtain higher strength hydrogel, the light duration can be extended as appropriate.
Explanation:
P477925 solution has thermally induced gel characteristics (heating up gel). During solution preparation, cooling is conducive to dissolution. It is recommended to let the solution dissolve at 2-8 ℃ and shake several times with a vortex mixer during this period.
P477925 solution with a concentration ≥ 20% will appear gel when it stands at room temperature. The higher the concentration is, the easier it is to gel. At this time, the gel is a physically reversible gel. When the temperature drops to 2~8 ℃, the solution will resume its state.
Kind reminder: Do not look directly at the curing light source.
Semi finished kit:
Component A: F127DA, white powder, 1g/bottle or 5g/bottle: stored in a dark place
Component B: Photoinitiator LAP, white powder, 0.05g/bottle * (1 or 5): stored in a dark place
规格
别名
Poloxamer F127 二丙烯酸酯 | 聚乙二醇
英文别名
Poloxamer F127 diacrylate, PEG-PPG-PEG
规格或纯度
平均Mw~15kDa
英文名称
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) diacrylate
储存条件
避光,-20°C储存,充氩
运输条件
超低温运输
名称和识别符
分子类型
聚合物
技术文档
高级数据
化学和物理性质
敏感性
对光线敏感；对湿度敏感；对空气敏感；对热敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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