计算溶液所需的质量、体积或浓度。
聚-L-赖氨酸三氟乙酸盐是一种带正电荷的合成聚氨基酸,每个赖氨酸单元有一个 TFA,它是一种可溶于水的结晶固体。 聚-L-赖氨酸 TFA 的应用包括与活性分子结合以提高活性、逐层沉积技术以及与核酸复合以进行基因表达。这种特殊的聚-L-赖氨酸三氟乙酸酯具有非 C-末端具有反应性烷基酰胺官能团,N-末端具有三苯甲基保护的硫醇或C-末端具有三苯甲基保护的硫醇。 使用 TFA/Et3SiH (98:2 v/v) 混合物可轻松切割三苯甲基。我们的聚氨基酸已通过透析纯化,在 0.2um 上无菌过滤,冻干并储存在 Ar 下。
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Poly-L-lysine trifluoroacetate is a positively charged synthetic polyamino acid having one TFA per lysine unit. It is a crystalline solid soluble in water. Applications for poly-L-lysine TFA include the conjugation to active molecules for improved activities, the layer-by-layer deposition techniques, and the complexation with nucleic acids for gene expression.This particular poly-L-lysine trifluoroacetate has either a non-reactive alkylamide function on the C-terminal and a trityl protected thiol on the N-terminal or a trityl protected thiol on the C-terminal. The trityl group is easily cleavable using a TFA/Et3SiH (98:2 v/v) mixture.Our polyamino acid has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar.
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