计算溶液所需的质量、体积或浓度。
产品描述:
在含有 1-4mM MgCl2,每种 dNTP 浓度为 200 μM,靶向 λ DNA 约 500 个碱基对区域的引物(浓度分别为 1.0μM),1ng/100μL 的 λ DNA 模板和 2.5 单位/100μL 的 Taq DNA 聚合酶的反应物中,以 1X 的终浓度(10mM Tris-HCl,pH 8.3,25°C,50mM Kcl)对 10X PCR 缓冲液 II 进行了测试。反应进行了 25 个循环,94℃ 双链 DNA 变性,55℃ 退火 DNA 片段和 72℃
产品应用:
不含 MgCl2的 10x PCR 缓冲液已与PCR 酶一起使用。不含MgCl2的10X PCR缓冲液已被用作聚合酶链反应(PCR)扩增混合物中的一种组分,用于霉菌毒素霉菌DNA、寄生虫DNA和尖孢镰刀菌DNA的扩增。
产品规格与参数:
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When 1-4mM MgCl2 is contained, the concentration of each dNTP is 200 μ M. Targeting λ Primers for about 500 base pair regions of DNA (concentration is 1.0 respectively μ M),1ng/100 μ L's λ DNA template and 2.5 units/100 μ In the reactant of Taq DNA polymerase L, 10X PCR buffer II was tested with 1X final concentration (10mM Tris HCl, pH 8.3, 25 ° C, 50mM Kcl). The reaction was carried out for 25 cycles, double stranded DNA was denatured at 94 ℃, DNA fragments were annealed at 55 ℃ and 72 ℃
Product application:
The 10x PCR buffer without MgCl2 has been used with PCR enzyme. The 10X PCR buffer without MgCl2 has been used as a component in the PCR amplification mixture for the amplification of mycotoxin mold DNA, parasite DNA and Fusarium oxysporum DNA.
Product specifications and parameters:
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