SDS变性电泳上样缓冲液,4×（Protein SDS PAGE Loading Buffered Solution, 4×）适用于SDS-PAGE(SDS-变性聚丙烯酰胺凝胶电泳)时作蛋白质上样用。其主要成份为SDS，DTT，溴酚蓝，缓冲盐溶液等。SDS可与蛋白质结合使蛋白质-SDS复合物上带有大量的负电荷，这时蛋白质本身的电荷完全被SDS掩盖，消除了各种蛋白质本身电荷的差异；SDS还可以断开分子内和分子间的氢键，破坏蛋白质分子的二级和三级结构。DTT可以断开半胱氨酸残基之间的二硫键，破坏蛋白质结构，消除了蛋白结构之间的差异。最终无电荷及结构上差异的蛋白（亚单位），电泳迁移只是与其分子量大小有关。溴酚蓝用作电泳时的指示剂，可大概指示电泳结束的时间。

使用方法：

1.蛋白样品与上样缓冲液按3:1的比例稀释使用。如果蛋白样品浓度过高，可用三级水或一级水稀释。2.混匀后，100℃水浴（或金属浴）加热 5分钟，使蛋白变性。3.冷却至室温后，10000-14000rpm 离心 2-5 分钟，直接上样电泳即可。4.蛋白变性后若常温放置时间较长或经低温保存会出现蛋白复性现象，电泳前需重新加热变性。使用范围：适用于一般生物学及医学研究

产品规格与参数：

货号	中文品名	浓缩倍数	包装
P493126-01-10×1ml	SDS变性电泳上样缓冲液	4×	10×1ml
P493126-01-1ml	SDS变性电泳上样缓冲液	4×	1ml
P493126-01-2×1ml	SDS变性电泳上样缓冲液	4×	2×1ml
P493126-02-10×1ml	SDS变性电泳上样缓冲液	5×	10×1ml
P493126-02-1ml	SDS变性电泳上样缓冲液	5×	1ml
P493126-02-2×1ml	SDS变性电泳上样缓冲液	5×	2×1ml
P493126-03-10×1ml	SDS变性电泳上样缓冲液	6×	10×1ml
P493126-03-1ml	SDS变性电泳上样缓冲液	6×	1ml
P493126-03-5×1ml	SDS变性电泳上样缓冲液	6×	5×1ml

Product introduction:

SDS denaturing electrophoresis loading buffer, 4 × （Protein SDS PAGE Loading Buffered Solution, 4 ×） It is suitable for protein loading during SDS-PAGE (SDS denatured polyacrylamide gel electrophoresis). Its main ingredients are SDS, DTT, bromophenol blue, buffer salt solution, etc. SDS can combine with protein to make the protein SDS complex carry a large number of negative charges. At this time, the charge of the protein itself is completely covered by SDS, eliminating the difference in the charge of various proteins; SDS can also break the intramolecular and intermolecular hydrogen bonds and destroy the secondary and tertiary structures of protein molecules. DTT can break the disulfide bond between cysteine residues, destroy the protein structure, and eliminate the differences between protein structures. The electrophoretic migration of proteins (subunits) with no charge and structural differences is only related to their molecular weight. Bromophenol blue is used as an indicator for electrophoresis, which can roughly indicate the end time of electrophoresis.

usage method:

1. Dilute the protein sample and the sample buffer solution at a ratio of 3:1 for use. If the concentration of protein sample is too high, it can be diluted with Grade III water or Grade I water. 2. After mixing, heat it in 100 ℃ water bath (or metal bath) for 5 minutes to denature the protein. 3. After cooling to room temperature, centrifugate at 10000-14000 rpm for 2-5 minutes, and then directly load samples for electrophoresis. 4. After protein denaturation, if the protein is stored at room temperature for a long time or stored at low temperature, protein renaturation will occur, and it needs to be reheated for denaturation before electrophoresis. Scope of application: applicable to general biological and medical research

Product specifications and parameters:

SKU	Name	Concentration multiple	Pack Size
P493126-01-10×1ml	Protein SDS PAGE Loading Buffered Solution	4×	10×1ml
P493126-01-1ml	Protein SDS PAGE Loading Buffered Solution	4×	1ml
P493126-01-2×1ml	Protein SDS PAGE Loading Buffered Solution	4×	2×1ml
P493126-02-10×1ml	Protein SDS PAGE Loading Buffered Solution	5×	10×1ml
P493126-02-1ml	Protein SDS PAGE Loading Buffered Solution	5×	1ml
P493126-02-2×1ml	Protein SDS PAGE Loading Buffered Solution	5×	2×1ml
P493126-03-10×1ml	Protein SDS PAGE Loading Buffered Solution	6×	10×1ml
P493126-03-1ml	Protein SDS PAGE Loading Buffered Solution	6×	1ml
P493126-03-5×1ml	Protein SDS PAGE Loading Buffered Solution	6×	5×1ml

规格

英文名称

Protein SDS PAGE Loading Buffered Solution

储存条件

-20°C储存

运输条件

超低温运输

名称和识别符

分子类型

生物试剂/缓冲液

技术文档

📋 安全数据表 (SDS)

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