E2627121计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP ONE liquid ready-to-use substrate is active and stable. PNPP ONE shows superior background characteristics due to its high temperature robustness compared to pNPP (cat. no. P494193).
Product Characteristics
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. Liberated p-nitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP ONE liquid, ready-to-use substrate is an active and stable substrate formulation. pNPP ONE shows superior background characteristics due to its high temperature robustness, compared to pNPP (cat. no. P494193) making it suitable for use in hot climate countries.
Composition & Properties
pNPP ONE is a liquid, ready-to-use and aqueous formulation. The buffer solution contains stabilized
pNPP and appropriate stabilizing components. Recommended stop solution is an equal volume of 0.1 M sodium hydroxide solution.
Working Procedure
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark.
2.Recommended volumes are 100 µl substrate solution per microtiter well. Development time is typically 15- 30 minutes. Optimal time is determined by the user and will vary according to the procedure and the incubation temperature.
3.100 µl of 0.1 M NaOH is then added and mixed thoroughly.
4.In kinetic assays, the color is read directly at 405 nm. For endpoint assays the absorbance is read at 405/620 nm.
Tips & Tricks
• If a more sensitive substrate giving lower detection limits is needed, we recommend pNPP (cat no. P494193). This substrate offers a 10% higher sensitivity than pNPP ONE.
• A stop solution of 0.1 M NaOH requires no hazardous labelling. In case a higher assay sensitivity is required, a 1.0 M NaOH may be used as stop solution offering an approx. increase of OD of 3%. In both cases a 1 hour stop stability is guaranteed.
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light and heat.
• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.
规格
别名
对硝基苯酚
规格或纯度
Ready-to-use (RTU)
英文名称
pNPP ONE
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
图片
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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