K2315082计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
概述
Product Characteristics
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP, liquid ready-to-use substrate, is highly active compared to pNPP ONE (cat. No. P494181). The substrate gives superior results due to its sensitivity, allowing very low detection limits.
Composition & Properties
pNPP is a liquid, ready-to-use and aqueous formulation. The buffer solution contains stabilized pNPP and appropriate stabilizing components. Recommended stop solution is an equal volume of 0.1 M sodium hydroxide solution.
Working Procedure
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark.
2.Recommended volumes are 100 µl substrate solution per microtiter well. Development time is typically 15- 30 minutes. Optimal time is determined by the user and will vary according to the procedure and the incubation temperature.
3.100 µl of 0.1 M NaOH is then added and mixed thoroughly.
4.In kinetic assays, the color is read directly at 405 nm. For endpoint assays the absorbance is read at 405/620 nm.
Tips & Tricks
• For use in hot climate countries, due to superior background characteristics, we recommend pNPP ONE (cat. no. P494181)
• A stop solution of 0.1 M NaOH requires no hazardous labelling. In case a higher assay sensitivity is required, a 1.0 M NaOH may be used as stop solution offering an approx. increase of OD of 3%. In both cases a 1 hour stop stability is guaranteed.
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light and heat.
• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color.
Dispensing guidelines are available upon request.
规格
规格或纯度
Ready-to-use (RTU)
英文名称
pNPP
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
图片
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
引用文献
| 1. Zizhe Li, Liang Luo, Chaochao Chen, Qiang Li, Qiyuan Zhao, Ghulam Mujtaba Mari, Chen Xing, Xuefeng Xia, Jianzhong Shen, XueZhi Yu, Zhanhui Wang, Yantong Pan. (2025) Environment-friendly dual-mode immunoassay based on the inner filter effect of polydopamine-polyethylenimine copolymer nanoparticles for the highly sensitive detection of norfloxacin. FOOD CHEMISTRY, [PMID:40138822] [10.1016/j.foodchem.2025.143928] |
溶液计算器
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