计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本植物蛋白抽提试剂盒可从新鲜、冰冻或干燥植物组织中提取可溶性植物蛋白。适用 于多种植物及植物的不同部位（如根、茎、叶、花、种子等）的蛋白提取，提取效率高、 蛋白产量大、活性高、速度快。所提蛋白可直接进行蛋白电泳分析、免疫沉淀、Western Blot、蛋白活性测定及蛋白纯化等实验。所提蛋白可用BCA蛋白定量试剂盒进行浓度的测定。
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注意事项：
1．本品含有1mM EDTA。
2．为防止蛋白降解，所有的操作尽量在冰上进行。
3．使用本产品提取蛋白后，可采用BCA法进行蛋白定量。
4．为了获得实验最佳效果，请根据实验调整最佳使用量。
操作步骤：
1．请在蛋白抽提前取出实验所需Plant Protein Extraction Reagent进行预冷。
2．称量试验植物组织的重量。按照1 g组织加入5 ml Plant Protein Extraction Reagent（在蛋白 抽提前按照1:99比例加入Protease Inhibitor Cocktail）。
注意：
1）匀浆前将大块的植物组织剪成小块，机械匀浆器匀浆10 s，间歇10 s，反复三次进行裂解， 根据组织样本不同选用适合的匀浆方式。
2）裂解液的使用量依据植物不同部位进行调整，若需要浓缩的蛋白提取物，可适当减少Plant Protein Extraction Reagent使用量。
3．匀浆后冰上孵育20-30分钟。
4．4℃ 13,400×g，离心20分钟。
5．收集上清中的可溶性蛋白，进行下一步的纯化或下游分析。
This plant protein extraction kit can extract soluble plant proteins from fresh, frozen, or dried plant tissues. Suitable for protein extraction from various plants and different parts of plants (such as roots, stems, leaves, flowers, seeds, etc.), with high extraction efficiency, high protein yield, high activity, and fast speed. The extracted protein can be directly subjected to protein electrophoresis analysis, immunoprecipitation, Western Blot, protein activity determination, and protein purification experiments. The concentration of the extracted protein can be determined using the BCA protein quantification kit .
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Precautions：
1. This product contains 1mM EDTA.
2. To prevent protein degradation, all operations should be carried out on ice as much as possible.
3. After extracting protein using this product, the BCA method can be used for protein quantification.
4. To achieve the best experimental results, please adjust the optimal usage amount according to the experiment.
Operation steps:
1. Please remove the required Plant Protein Extraction Agent for pre cooling before protein extraction.
2. Weigh the weight of the experimental plant tissue. Add 5 ml of Plant Protein Extraction Agent to 1 g of tissue (add Protein Inhibitor Cocktail in a 1:99 ratio before protein extraction).
Attention:
1) Before homogenization, cut large pieces of plant tissue into small pieces and homogenize them with a mechanical homogenizer for 10 seconds, with an interval of 10 seconds. Repeat the process three times and select the appropriate homogenization method according to the different tissue samples.
2) The amount of lysate used is adjusted according to different parts of the plant. If concentrated protein extracts are needed, the amount of Plant Protein Extraction Agent used can be appropriately reduced.
3. After homogenization, incubate on ice for 20-30 minutes.
4.4 ℃ 13400 × g, centrifuge for 20 minutes.
5. Collect soluble proteins from the supernatant for further purification or downstream analysis.
规格
英文名称
Plant Protein Extraction Kit
储存与运输
储存条件
-20°C储存
运输条件
超低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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