计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP,0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取,或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水,即可制备活性底物溶液。片剂单独包装在铝箔包装中 。
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.