计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
棕榈酸BSA高脂细胞添加剂的有效成分是棕榈酸 (Palmitic acid),浓度是6 mM。本产品为无菌 (0.22μm 过滤) 溶液,可与完全培养基直接互溶用于实验。本产品所用溶剂不含NaOH或有机溶剂,对细胞无毒性。本产品可用于诱发细胞脂毒性、诱导内质网应激、氧化应激、线粒体损伤、细胞凋亡等。
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使用说明:
1、针对不同细胞种类及实验目的,本产品所用浓度不同,一般与细胞培养基按 1: 120-1: 10倍比稀释。
2、对于首次使用本产品,建议采用浓度梯度处理细胞,摸索针对特定细胞、达到特定 效果的适宜浓度。
3、待细胞生长至80-100%汇合度时,换成新鲜培养基,按适当浓度滴加本产品,对照组加入等量体积溶剂对照,轻柔混匀后将细胞放入细胞孵箱中继续培养。
4、加入本品20-48h后,收集细胞,进行下一步分析。
注意事项:
1、收到产品后可分装后于-20℃长期保存;可在2-8℃短期保存3个月。
2、由于溶剂 BSA 影响吸光度,使用CCK-8 (Aladdin货号C266180) 检测细胞毒性时,在弃掉高脂细胞添加剂与培养基混液后,需要用PBS清洗细胞2-3次,再加入CCK-8工作液。
The active ingredient of this product is palmitic acid at a concentration of 6 mM. It is a sterile solution filtered through a 0.22 μm membrane and can be directly mixed with complete culture medium for experimental use. The solvent adopted in this product contains neither sodium hydroxide nor organic solvents, thus exerting no cytotoxicity to cells. This supplement can be applied to induce cellular lipotoxicity, endoplasmic reticulum stress, oxidative stress, mitochondrial damage, cell apoptosis and other related cellular phenotypes.
Contents & Storage
| P767990 | Component | 2x5 mL | 2x10 mL | Storage |
| P767990A | Palmitic acid BSA high-fat cell additive | 5 mL | 10 mL | -20°C. Avoid freeze/thaw cycle |
| P767990B | Palmitic acid solvent control | 5 mL | 10 mL | -20°C. Avoid freeze/thaw cycle |
Instructions for Use
1.The working concentration of this product varies depending on cell types and experimental objectives. Generally, dilute it with cell culture medium at a volume ratio ranging from 1:120 to 1:10.
2.For first-time use, treat cells with a concentration gradient to identify the optimal concentration that yields the desired effect for your specific cell line.
3.When cells reach 80–100% confluence, replace with fresh culture medium. Add this product dropwise at the appropriate dilution ratio. Add an equal volume of vehicle control to the control group, mix gently, and place the cells back into the incubator for further incubation.
4.Harvest cells 20–48 hours after treatment for subsequent analysis.
Precautions
Upon receipt, aliquot the product for long-term storage at -20 °C; short-term storage at 2–8 °C is acceptable for up to 3 months.
The solvent containing BSA may interfere with absorbance readings. When measuring cytotoxicity via CCK-8 (Aladdin Cat. No. C266180), aspirate the mixture of high-fat cell supplement and culture medium, wash the cells 2–3 times with PBS, then add CCK-8 working solution.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 | |
| 分析证书 | P767990 |
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