脑脊液（CerebroSpinal Fluid，CSF）是存在于脑室、蛛网膜下腔和脊髓中央管内的无色透明液体，由脑室中的脉络丛产生，与血浆和淋巴液的性质相似，正常成年人的脑脊液约100~150mL，弱碱性，不含红细胞，具有一定的化学成分和压力，对维持颅压的相对稳定有重要作用，当中枢神经系统受损时，脑脊液的检测成为重要的辅助诊断手段。
检测原理：脑脊液中球蛋白与苯酚结合，可形成不溶性蛋白盐而下沉，产生白色浑浊或沉淀，该检测法又称潘氏（Pandy）实验，多用于人或动物脑脊液的蛋白定性检查。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）
1、取小试管，加入2~3mL Pandy Reagent。
2、滴加脑脊液1～2滴（约0.05～0.1mL），混匀，黑色背景下立即观察。
结果判断
阴性 | 清晰透明，不显雾状 |
极弱阳性（±） | 微呈白雾状，在黑色背景下才可见。 |
阳性（+） | 灰白色云雾状 |
（2+） | 白色浑浊 |
（3+） | 白色浓絮状沉淀 |
（4+） | 白色凝块 |
注意事项
1、样本收集后应立即送检，一般不应超过1h。
2、正常时多为阴性或极弱阳性，有脑组织和脑脊髓疾患时呈阳性反应。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Cerebrospinal Fluid (CSF) is a colorless, transparent liquid found in the ventricles of the brain, the subarachnoid space, and the central canal of the spinal cord. It is produced by the choroid plexus in the ventricles and has properties similar to plasma and lymph. A normal adult has approximately 100–150 mL of CSF, which is slightly alkaline, contains no red blood cells, and has specific chemical components and pressure. It plays an important role in maintaining the relative stability of intracranial pressure. When the central nervous system is damaged, CSF testing becomes an important auxiliary diagnostic tool.
Detection Principle
Globulins in cerebrospinal fluid bind with phenol to form insoluble protein salts, which precipitate and produce a white turbidity or sediment. This test is also known as the Pandy test and is commonly used for qualitative protein testing in human or animal cerebrospinal fluid. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.
Operating Steps (For Reference Only)
1. Take a small test tube and add 2–3 mL of Pandy Reagent.
2. Add 1–2 drops (approximately 0.05–0.1 mL) of cerebrospinal fluid, mix well, and immediately observe against a black background.
Result Interpretation
Negative | Clear and transparent, no haze. |
Very Weakly Positive (±) | Slight white haze, visible only against a black background. |
Positive (+) | Grayish-white, cloudy. |
(2+) | White turbidity. |
(3+) | White, thick flocculent precipitate. |
(4+) | White coagulum. |
Precautions
1. Samples should be sent for testing immediately after collection, generally within 1 hour.
2. Normally, results are mostly negative or very weakly positive. Positive reactions may indicate brain or cerebrospinal disorders.
3. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
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