计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
|
产品简介
本产品是利用细胞内外盐离子浓度差可导致细胞膜胀破的原理来裂解细胞，释放 基因组DNA，无需提取纯化基因组DNA。该产品适用于多种来源的样本，样本处理之 后可直接作为模板进行PCR及qPCR实验，能达到纯化后DNA作为模板进行PCR及 qPCR实验的效果。
使用方法
1. 根据不同的样本类型,按照下表准备相应的样本用量。
2.将样本加入到1.5mi离心管中，加入下表推荐体积的Solution A.涡旋涉20s,并按照下表推荐的处理方法,室温静置3-5min,或95°C金属浴振荡育3-5min。
3.
样本充分裂解后(95°C金属浴振荡孵育的样本需恢复室温),加入下表推荐体积的Solution B，并涡旋震荡30s。
4.
处理后的样本如在2小时内进行下一步试验，请于4℃
保存，如不能立即进行下一步试验，请于-20℃
保存。
1)根据实验条件的需求，可以扩大或减小样本用量，并等比例增加Solution A及Solution B的用量, 2)血液和细胞样本，室温裂解的温度要求在25C左右，如环境温度达不到25°，可适当延长裂解时间，或延长涡旋震荡时间，保证样品充分裂解。如没有相关专业仪器，可剧烈晃动离心管，以保证充分裂解。 3)组织样本加入10倍体积的生理盐水制成组织匀浆液后，可按照血液样本的处理方法进行处理。 4)严禁使用过期产品，不同试剂请勿混用。 5)实验用品应定期清洁并用10%的84消毒液或紫外灯进行防污染处理，专区专用，禁止交叉使用，以免污染，检测结束后，应立即对工作台进行清洁。 |
Products content
Products Introduction
This product uses the principle that the difference between the concentration of salt ions inside and outside the cell can cause the cell membrane to burst to lyses the cell and releases the genomic DNA, without the need of extracting and purifying the genomic DNA.This product is suitable for a variety of sources of samples, and can be used as a template for PCR and qPCR experiments after sample processing, and can achieve the effect of the purified DNA used as a template for PCR and qPCR experiments.
Usage
1. Depending on the type of sample, prepare the appropriate sample size according to the table below.
2. Add the sample to a 1.5-mi centrifuge tube and add the recommended volume of Solution A as shown in the table below. Vortex for 20 s and allow to stand at room temperature for 3-5 min or incubate in a metal bath at 95°C for 3-5 min as recommended in the table below.
3. After the sample has been sufficiently lysed (samples incubated in a metal bath at 95°C should be brought to room temperature), add the recommended volume of Solution B as shown in the table below and vortex for 30s.
4. Store processed samples at 4°C if the next test is to be performed within 2 hours, or at -20°C if the next test cannot be performed immediately.
take note of
1) Depending on the requirements of the experimental conditions, the amount of samples can be expanded or reduced, and the amount of Solution A and Solution B can be increased in equal proportions.
2) For blood and cell samples, the temperature of room temperature lysis is required to be around 25C. If the ambient temperature does not reach 25°, the lysis time can be extended appropriately, or the vortex shaking time can be extended to ensure that the samples are fully lysed. If there is no relevant professional instrument, the centrifuge tube can be shaken vigorously to ensure adequate lysis.
3) After the tissue sample is made into tissue homogenate by adding 10 times the volume of saline, it can be processed in the same way as blood samples.
4) Strictly prohibit the use of expired products, please do not mix different reagents.
5) laboratory supplies should be regularly cleaned and 10% of the 84 disinfectant solution or ultraviolet lamp for anti-pollution treatment, special areas dedicated to prohibit cross use, so as to avoid contamination, the end of the test, the bench should be cleaned immediately.
规格
别名
快速DNA提取试剂盒
英文名称
Quick DNA Extraction Kit
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
试剂盒
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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