计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,干燥,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purine nucleoside phosphorylase (PNP) is a key enzyme in purine metabolism, which is involved in the purine rescue pathway. The deficiency of Purine nucleoside phosphorylase resulted in impaired T cell function. In the presence of inorganic orthophosphate as the second substrate, Purine nucleoside phosphorylase catalyzes the breaking of the glycosidic bond between ribose and deoxyribonucleoside to generate purine base and ribose (deoxyribose) -1-phosphate.
Reaction

Product description
Appearance: White amorphous powder
Source: Microorganism
Enzyme Commission Number: EC 2.4.2.1
CAS Number: 9030-21-1
Storage temperature: -20℃
Activity: ≥40U/mg enzyme powder; ≥200U/mg protein
Unit definition: One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.
Properties
| Stability: | Stable at -20℃ for at least five years | |
| Molecular weight: | 32 kDa (SDS-PAGE) | |
| Isoelectric point: | 6.0 | |
| Michaelis constant: | 2.2×10⁻⁴M (Inosine) | |
| Optimum pH: | 7.5-8.0 | {Fig. 1} |
| Optimum temperature: | 60℃ | {Fig. 3} |
| pH Stability: | 6.0-9.0 (30℃, 16hr) | {Fig. 2} |
| Thermal stability: | < 55℃ (pH 7.7, 30min) | {Fig. 4} |
| Inhibitors: | Cu²⁺,NEM,Proclin,SDS | |
| Effect of various chemicals: | {Table 1} |
Table 1. Effect of Various Chemicals on PNP
[The enzyme dissolved in 50mM K-phosphate buffer, pH 7.5 (10U/ml) was incubated with each chemical at 37℃ for 2hr.]
.png)
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 High Performance 级别指南 → 查看 EnzymoPure™ 级别指南 →