甘油激酶 (GK)

Name: 甘油激酶 (GK)
SKU: R1507822
Availability: InStock

CAS: 9030-66-4 货号: R1507822 EC号: 232-862-0 PubChem CID: 135333386

27篇引用文献 COA

有货

级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? 来源于脑膜脓毒性黄杆菌; ≥70 U/mg enzyme powder

Accession #

Q7M0X5

生物活性

≥70 U/mg enzyme powder

规格

库存

价格

数量

1KU

R1507822-1KU

期货

¥699.90

5KU

R1507822-5KU

期货

¥1,699.90

25KU

R1507822-25KU

期货

¥4,999.90

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概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 27 篇同行评审文献引用。

概述

Glycerol Kinase (GK) acts as a NR4A1 inhibitor with enzymatic activity. It directly binds to and inhibits the transcription factor NR4A1, thereby negatively regulating hepatic gluconeogenesis and reducing blood glucose levels. Glycerol Kinase (GK) positively regulates UCP1 expression via partial dependence on the β-adrenergic receptor-cAMP-CREB pathway, promotes browning of white adipose tissue and thermogenesis, and further modulates intracellular fatty acid composition and energy metabolism. In diabetic mouse models, overexpression of Glycerol kinase effectively antagonizes NR4A1-induced hyperglycemia, demonstrating potential for improving glucose homeostasis. Glycerol Kinase (GK) can be used for studies on diabetes and obesit.

Glycerol + ATP ⟶ L-α-Glycerolphosphate + ADP

Advantages

① Thermal stability

② Stability in solution

③ Antiseptic stability

Properties

Substrate specificity	See Table 1	-
Molecular weight	150 kDa (TSK G3000SWXL) 50 kDa (SDS-PAGE)	-
Isoelectric point	pH 4.3	-
Michaelis constants	Glycerol 8.8 × 10⁻⁵ MATP 3.0 × 10⁻⁵ M	-
Optimum pH	8.0	Figure 1
pH stability	5.0–11.0 (37°C, 60 min)	Figure 2
Optimum temperature	80°C (Tris-HCl buffer)	Figure 3
Thermal stability	Stable at 60°C and below (pH 6.5, 10 min)Comparison data between GKZ and GK	Figure 4

Applications

This enzyme is useful for enzymatic determination of triglyceride.

Assay

● Principle

The assay is based on the increase in absorbance at 366 nm as NADH is produced in the following reactions:

ATP: Adenosine triphosphate

NAD: Nicotineamido adenine dinucleotide

G3PDH: Glycerol-3-phosphate dehydrogenase

● Unit definition

One unit is defined as the amount of enzyme which converts 1 μmole of glycerol to glycerol-3-phosphate per minute at 37℃ under the conditions specified in the assay procedure.

● Reagents

1. Substrate-coenzyme mixture

80 mM ATP solution pH 7.0　　　　　　2.0 ml

0.1 M NAD solution　　　　　　　　　 2.0 ml

0.1 M Glycerol solution　　　　　　 2.0 ml

2. Buffer solution

Glycine-hydrazine buffer containing 1.5mM MgCl₂ pH 9.8

Dissolve 12.5g hydrazine hydrate and 1.5g glycine with about 80ml distilled water. Add 1.5ml 0.1M MgCl₂ solution and adjust pH to 9.8 with 1N HCl at 25℃. Add distilled water to make a total of 100ml.

3. G3PDH solution

Use G3PDH (NH₄)₂SO₄ suspension (undiluted)

4. Enzyme dilution buffer

10 mM KH₂PO₄-NaOH buffer pH 7.0 containing 10 mM glycerol

● Enzyme solution

Accurately weigh about 20 mg of the sample and add enzyme dilution buffer to make a total of 20 ml. Dilute it with enzyme dilution buffer to adjust the concentration as required.

● Procedure

1. Pipette accurately 1.80 ml of buffer into a small test tube, add 0.15 ml of substrate/coenzyme mixture and 20 μl of G3PDH, mix immediately.After mixing, preincubate at 37℃.

2. After 5 min, add 30 μl of enzyme solution and mix to start the reaction at 37℃.※ In the case of a test blank, add 30 μl of enzyme dilution buffer in place of enzyme solution.

3. After starting the reaction, measure the rate of increase per minute in absorbance at 366 nm. The rate must be measured within the linear portion of the absorbance curve.

Absorbance

sample : As/min

blank : Ab/min

△A/min = (As/min−Ab/min) ≤0.050 Abs/min

● Calculation

Activity (U/mg of powder) = {△A/min}/3.3 × 2.00/0.03 × 1/x

3.3 : millimolar extinction coefficient of NADH at 366 nm (cm²/μmole)

2.00 : final volume (ml)

0.03 : volume of enzyme solution (ml)

X : concentration of the sample in enzyme solution (mg/ml)

规格

产品名称

甘油激酶 (GK)

别名

ATP：甘油3-磷酸转移酶

英文别名

ATP:glycerol 3-phosphotransferase | glycerol kinase | glycerokinase | glpK

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, 来源于脑膜脓毒性黄杆菌; ≥70 U/mg enzyme powder

生物活性

≥70 U/mg enzyme powder

种属

脑膜炎脓杆菌(Flavobacterium meningosepticum)

来源

重组表达

SDS-PAGE

50 kDa

CAS编号和信息

9030-66-4

酶学委员会编号

EC 2.7.1.30

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

来源于脑膜脓毒性黄杆菌; ≥70 U/mg enzyme powder

复溶

Reconstitute in 10 mM KH2PO4–NaOH buffer pH 7.0 containing 10 mM glycerol.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃；复溶后建议分装，避免反复冻融。

单位定义

One unit is defined as the amount of enzyme which converts 1 μmole of glycerol to glycerol–3–phosphate per minute at 37℃ under the conditions specified in the assay procedure.