在生物科研领域,经常需要去除红细胞。去除红细胞的方法有ACK Lysis Buffer、Tris-氯化铵红细胞裂解液、Gey's Lysis Buffer等,在裂解红细胞之前,可以采用PBS清洗红细胞,亦可用专门的红细胞洗涤液,以便充分清洗细胞。红细胞洗涤液 (pH 7.2)为无菌溶液,主要由磷酸盐、氯化钠、氯化钾等组成。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、取新鲜抗凝血,1000g离心5min,去除血浆。
2、 加入5倍细胞沉淀体积的红细胞洗涤液 (pH7.2),清洗2-3次。
注意事项:
1、后续试验如果是用于细胞培养,操作过程中应注意无菌操作,尽量在超净工作台内操作。
2、离心步骤尽量在4℃离心机上操作。
3、离心洗涤后,通常极微量的红细胞不会影响后续的检测。
4、试剂开封后请尽快使用,以防影响后续实验效果。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Product Introduction
In the field of biological research, red blood cell removal is often required. Common methods for red blood cell removal include using ACK Lysis Buffer, Tris-ammonium chloride red blood cell lysis buffer, Gey's Lysis Buffer, etc. Before lysing red blood cells, PBS can be used to wash the red blood cells, or a dedicated red blood cell washing solution can be applied to ensure thorough cell cleaning.
Red Blood Cell Washing Solution (pH 7.2) is a sterile solution mainly composed of phosphate, sodium chloride, potassium chloride, and other components. This reagent is for research use only and not intended for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only)
1. Take fresh anticoagulated blood, centrifuge at 1000×g for 5 minutes, and discard the plasma.
2. Add red blood cell washing solution (pH 7.2) in a volume 5 times that of the cell pellet, and wash the cells for 2–3 times.
Precautions
1. If the subsequent experiment involves cell culture, strict aseptic technique should be followed during the operation, preferably performed in a laminar flow hood.
2. Centrifugation steps should be carried out in a centrifuge pre-cooled to 4°C as much as possible.
3. After centrifugation and washing, trace amounts of residual red blood cells generally will not interfere with subsequent assays.
4. Please use the reagent as soon as possible after opening to avoid compromising the results of subsequent experiments.
5. For your safety and health, wear a lab coat and disposable gloves during operation.