Ringer's液 (哺乳动物专用)又称林格氏液、复方氯化钠溶液,属于平衡盐溶液 (Balanced Salt Solution, BSS)的一种,BBS与细胞生长状态下的pH值、渗透压等环境状态一致,具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用,可满足体外实验中细胞生存并维持一定代谢的基本需要。
Ringer's Solution由0.86%氯化钠以及微量氯化钾和氯化钙组成,不含葡萄糖,用于哺乳动物 (或人)细胞离体实验,肌体灌流、清洗组织,并维持离体组织的正常生理功能。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
根据实验具体要求,保存或清洗离体组织。
注意事项:
1、由于Ringer's Solution含钙离子,容易产生沉淀。如果产生少许沉淀,可以加热溶解或过滤后使用,产生大量沉淀应弃用。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Ringer's Solution (Mammal-specific), also known as Ringer’s Solution or Compound Sodium Chloride Solution, is a type of Balanced Salt Solution (BSS). BSS matches the environmental conditions such as pH value and osmotic pressure required for cell growth. It functions to maintain osmotic pressure, regulate acid-base balance, and supply essential inorganic salt components for cell survival and metabolism, thus meeting the basic needs of cell survival and sustained metabolism in in vitro experiments.
Ringer's Solution consists of 0.86% sodium chloride, plus trace amounts of potassium chloride and calcium chloride, and contains no glucose. It is applicable to in vitro experiments on mammalian (or human) cells, tissue perfusion, tissue washing, and maintenance of the normal physiological functions of isolated tissues. This reagent is for research purposes only and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only)
Preserve or wash the isolated tissues in accordance with the specific requirements of the experiment.
Precautions
1. Ringer's Solution contains calcium ions and is prone to precipitation. If a small amount of precipitate forms, the solution can be heated to dissolve the precipitate or filtered before use. Discard the solution if a large amount of precipitate appears.
2. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
3. For your safety and health, wear a lab coat and disposable gloves during operation.