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抗性淀粉（RS）是指在人小肠内不能被酶解的淀粉，在大肠部分或完全发酵。RS是总膳食纤维的组分之一。 抗性淀粉的测定方法：样品使用胰a-淀粉酶和淀粉葡萄糖苷酶（AMG）37℃振荡水浴孵育16小时，在这期间，通过两种酶的联合作用，非抗性淀粉被水解成D-葡萄糖，孵育结束后，加入等体积的乙醇或工业甲基化酒精（IMS,变性乙醇）终止反应。离心上述溶液，收集上清勿弃，底部残留絮状团即为样品中的RS，用含水的IMS或50%乙醇(v/v)洗涤絮状团，洗涤后离心，再重复一次洗涤离心，收集离心后获得的上清，与之前收集的上清混合。小心倒出试管残留的液体，将絮状团置于冰水浴中，加入2M KOH溶解，溶解的同时用磁力搅拌机剧烈搅拌。用醋酸盐缓冲液将这个溶液调至中性，用AMG将淀粉定量水解成葡萄糖。D-葡萄糖用葡萄糖氧化酶/过氧化物酶试剂（GOPOD）测定，这也是对样品中RS含量的测定。非抗性淀粉（可溶性淀粉）的测定可通过集中的上清液并定容至100mL，再用GOPOD测定D-葡萄糖完成。
Resistant starch (RS) refers to starch that cannot be digested by enzymes in the human small intestine and is partially or completely fermented in the large intestine. RS is a component of total dietary fiber. Determination method for resistant starch: The sample is incubated with pancreatic α‑amylase and amyloglucosidase (AMG) in a shaking water bath at 37 °C for 16 hours. During this period, non‑resistant starch is hydrolyzed to D‑glucose by the combined action of the two enzymes. After incubation, an equal volume of ethanol or industrial methylated spirit (IMS, denatured ethanol) is added to terminate the reaction. The solution is centrifuged, and the supernatant is collected and retained. The residue at the bottom (a flocculent pellet) is the RS in the sample. The pellet is washed with aqueous IMS or 50 % (v/v) ethanol, centrifuged, and the washing/centrifugation step is repeated once. The supernatants obtained from the washing steps are combined with the previously collected supernatant. Any remaining liquid in the tube is carefully decanted, and the pellet is placed in an ice‑water bath and dissolved in 2 M KOH with vigorous stirring using a magnetic stirrer. The solution is then neutralized with acetate buffer, and the starch is quantitatively hydrolyzed to glucose by AMG. D‑Glucose is measured using glucose oxidase/peroxidase reagent (GOPOD), which gives the RS content in the sample. Non‑resistant starch (soluble starch) can be determined by pooling the combined supernatants, making up to 100 mL, and measuring D‑glucose with GOPOD.
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