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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
1,5-二磷酸核酮糖羧化/加氧酶（Rubisco, EC 4.1.1.39）是植物光合作用中的一个关键酶，既控制着CO₂的固定，同时又制约着碳素向 Calvin 循环和光呼吸循环分流，其活性直接影响着光合速率。本试剂盒检测原理是在 Rubisco 的催化下，1 分子的核酮糖-1,5-二磷酸（RuBP）与1 分子的CO₂结合，产生 2 分子的 3-磷酸甘油酸（PGA）；PGA 可通过外加的 3-磷酸甘油酸激酶和甘油醛-3-磷酸脱氢酶的作用，产生甘油醛-3-磷酸，伴随着 NADH 氧化生成NAD⁺；在 340 nm NADH 有特征吸收峰，而NAD⁺没有此吸收峰，测定340 nm 吸光度下降速率可以代表 Rubisco 的羧化酶活性。
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is a key enzyme in plant photosynthesis. It not only controls the fixation of CO₂ but also regulates the partitioning of carbon into the Calvin cycle and the photorespiration pathway, with its activity directly impacting the photosynthetic rate. The detection principle of this kit is based on the Rubisco-catalyzed reaction, where one molecule of ribulose-1,5-bisphosphate (RuBP) combines with one molecule of CO₂ to produce two molecules of 3-phosphoglycerate (PGA). Through the subsequent action of externally added 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, PGA is converted to glyceraldehyde-3-phosphate, accompanied by the oxidation of NADH to NAD⁺. NADH has a characteristic absorption peak at 340 nm, whereas NAD⁺ does not. Therefore, the rate of decrease in absorbance at 340 nm can be used to represent the carboxylase activity of Rubisco.
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