RPMI 1640 培养基由 Moore 等人于 1967 年在美国纽约州布法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发。其中“RPMI”为该研究所开发的系列培养基统称，“1640”为特定产品编号。该培养基是 McCoy's 5A 的改良型，采用碳酸氢盐缓冲系统，与多数哺乳动物细胞培养基不同，其原始配方设定的 pH 约为 8.0。RPMI 1640 最初专为淋巴细胞培养设计，如今已广泛用于各类正常细胞和癌细胞（尤其是悬浮细胞）的培养，是全球应用最广的基础培养基之一。本产品不含酚红。酚红通常用作 pH 指示剂（低 pH 呈黄色，高 pH 呈紫色，pH 7.2～7.4 呈红色，最适细胞生长），但因其可能模拟雌激素作用（影响雌激素敏感细胞）、干扰流式细胞术检测及影响无血清培养基中的钠‑钾平衡，本配方特意去除该成分，以适用更广泛的实验需求。本产品主要成分浓度如下： D‑葡萄糖：4000 mg/L L‑谷氨酰胺：600 mg/L 碳酸氢钠：2000 mg/L 产品最终 pH 值为 7.0～7.4。本产品含有细胞培养所需的多种氨基酸、维生素、无机盐等营养成分，但不含蛋白质、脂类或任何生长因子，因此使用时需额外添加血清或无血清添加物，以满足细胞生长需求。注意事项 1、本产品已过滤除菌，使用时请严格无菌操作，防止污染。 2、为保持最佳使用效果，请勿反复冻融。 3、仅限科研用途（或用于进一步研究），不得用于诊断或治疗。

RPMI 1640 medium was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Buffalo, New York, USA. In this designation, "RPMI" refers to the series of media developed by the Institute, while "1640" denotes the specific product catalog number. This medium is a modified version of McCoy's 5A, utilizes a bicarbonate buffering system, and—unlike most mammalian cell culture media—was originally formulated to a pH of approximately 8.0. Initially designed specifically for lymphocyte culture, RPMI 1640 has now been widely adopted for the cultivation of various normal and cancer cells (particularly suspension cells) and remains one of the most extensively used basal media worldwide. This product is formulated without phenol red. Phenol red is commonly employed as a pH indicator (appearing yellow at low pH, purple at high pH, and red at pH 7.2–7.4, which is optimal for cell growth). However, because it may mimic estrogenic effects (influencing estrogen‑sensitive cells), interfere with flow cytometric analysis, and affect sodium‑potassium balance in serum‑free media, this formulation deliberately omits the component to accommodate a broader range of experimental requirements. The concentrations of major components in this product are as follows: D‑Glucose: 4000 mg/L L‑Glutamine: 600 mg/L Sodium Bicarbonate: 2000 mg/L The final pH of the product is 7.0–7.4. This product contains various amino acids, vitamins, inorganic salts, and other nutrients required for cell culture; however, it does not contain proteins, lipids, or any growth factors. Therefore, supplementation with serum or serum‑free additives is necessary during use to meet cellular growth requirements. Precautions 1. This product has been sterilized by filtration. Please strictly adhere to aseptic techniques during handling to prevent contamination. 2. To maintain optimal performance, avoid repeated freeze‑thaw cycles. 3. For research use only (or for further investigative purposes); not intended for diagnostic or therapeutic applications.