RPMI 1640 培养基由 Moore 等人于 1967 年在美国纽约州法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发。其中“RPMI”为该研究所开发的系列培养基统称，“1640”为特定产品代号。该培养基是 McCoy's 5A 的改良型，采用碳酸氢盐缓冲系统，与多数哺乳动物细胞培养基不同，其原始配方设定 pH 约为 8.0。RPMI 1640 最初专为淋巴细胞培养设计，现已广泛应用于各类正常细胞和癌细胞（尤其悬浮细胞）的培养，是全球使用最广泛的基础培养基之一。本产品含细胞培养所需的多种氨基酸、维生素、无机盐等营养成分，并添加了以下组分： HEPES 缓冲剂（25 mM）：对细胞无毒性，可长时间维持稳定的 pH 范围，有效防止培养液 pH 波动对细胞生长的不利影响。青霉素‑链霉素双抗（青霉素 100 U/mL，链霉素 100 μg/mL）：联合使用可有效预防绝大多数细菌污染（青霉素作用于革兰氏阳性菌，链霉素对革兰氏阴性菌和阳性菌均有效，尤对阴性菌作用更强）。其他主要成分：D‑葡萄糖 2000 mg/L，L‑谷氨酰胺 300 mg/L，碳酸氢钠 2000 mg/L。本产品不含蛋白质、脂类或任何生长因子，使用时需额外添加血清或无血清添加物，以满足细胞生长需求。产品最终 pH 值为 7.0～7.2。注意事项 1、本产品已过滤除菌，使用时请严格无菌操作，避免污染。 2、为保持最佳使用效果，请勿反复冻融。 3、本产品仅限科研使用（或用于进一步研究），不得用于诊断或治疗。

RPMI 1640 medium was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Buffalo, New York, USA. In this nomenclature, "RPMI" refers to the series of media developed by the Institute, and "1640" denotes the specific product code. This medium is a modified version of McCoy's 5A, utilizes a bicarbonate buffering system, and—unlike most mammalian cell culture media—was originally formulated to a pH of approximately 8.0. Initially designed specifically for lymphocyte culture, RPMI 1640 has now been widely adopted for the cultivation of various normal and cancer cells (particularly suspension cells) and remains one of the most extensively used basal media worldwide. This product contains various amino acids, vitamins, inorganic salts, and other nutrients required for cell culture, and is supplemented with the following components: 1. HEPES buffer (25 mM): Non‑cytotoxic to cells, it maintains a stable pH range over extended periods, effectively preventing adverse effects on cell growth caused by pH fluctuations in the culture medium. 2. Penicillin‑streptomycin dual antibiotics (penicillin 100 U/mL, streptomycin 100 μg/mL): The combined use effectively prevents the vast majority of bacterial contamination (penicillin acts against Gram‑positive bacteria, while streptomycin is effective against both Gram‑negative and Gram‑positive bacteria, with stronger activity against the former). 3. Other major components: D‑Glucose 2000 mg/L, L‑Glutamine 300 mg/L, Sodium Bicarbonate 2000 mg/L. This product contains no proteins, lipids, or any growth factors; therefore, supplementation with serum or serum‑free additives is necessary during use to meet cellular growth requirements. The final pH of the product is 7.0–7.2. Precautions 1. This product has been sterilized by filtration. Please strictly adhere to aseptic techniques during handling to avoid contamination. 2. To maintain optimal performance, avoid repeated freeze‑thaw cycles. 3. This product is for research use only (or for further investigative purposes) and is not intended for diagnostic or therapeutic applications.