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RPMI 1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所(Roswell Park Memorial Institute, RPMI)开发出来的,RPMI是该研究所开发的一类细胞培养基,1640是培养基代号。RPMI 1640是改进型的McCoy's 5A培养基,使用碳酸氢盐缓冲系统,与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI 1640培养基最初是为淋巴细胞培养专门设计的,现在已广泛应用于各种正常细胞和癌细胞的培养,尤其是悬浮细胞的培养,是使用最为广泛的培养基之一。 注意事项 1、本产品经过滤除菌,使用时应注意无菌操作,避免污染; 2、为保持本产品的最佳使用效果,请勿进行冻融处理; 3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
RPMI 1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Buffalo, New York, USA. RPMI designates a series of cell culture media developed by this institute, and 1640 is the medium code. RPMI 1640 is a modified formulation of McCoy's 5A medium, utilizing a bicarbonate buffering system. Unlike most mammalian cell culture media, it is typically formulated at pH 8.0. Originally designed specifically for lymphocyte culture, RPMI 1640 is now widely used for the cultivation of various normal and cancer cells, particularly suspension cells, and has become one of the most commonly employed media. Precautions ① This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. ② To maintain optimal performance, avoid freeze‑thaw cycles. ③ This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.