RPMI（ Roswell Park Memorial Institute） 1640培养基最初开发用于培养悬浮和单层的人类白血病细胞。RPMI 1640培养基现已经被发现适合多种哺乳动物细胞，包括海拉，Jurkat，MCF-7，PC12，PBMC，星形胶质细胞和癌细胞。RPMI 1640培养基独特于其他培养基，因为它包含还原剂谷胱甘肽和高浓度的维生素。RPMI 1640培养基中含有生物素、维生素B12和PABA，而MEM培养基或DMEM培养基中不含这些成分，此外，其维生素肌醇、胆碱含量非常高。RPMI 1640培养基中不含蛋白质,脂质，或生长因子，因此需要补充其他试剂，通常补充10%胎牛血清。RPMI 1640培养基中使用碳酸氢钠缓冲体系(2.0 g / L)，同时需要5 - 10%的二氧化碳环境维持生理pH值。
本产品不含抗生素或抗真菌成分。
本产品含10mM HEPES，4500mg/L D-葡萄糖，292mg/L L-谷氨酰胺，110mg/L丙酮酸钠，1500mg/L碳酸氢钠。pH值7.0-7.2。
注意事项
1、本产品经过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
RPMI (Roswell Park Memorial Institute) 1640 medium was originally developed for culturing human leukemia cells in suspension and monolayer. It has since been found suitable for a wide variety of mammalian cells, including HeLa, Jurkat, MCF‑7, PC12, PBMCs, astrocytes, and cancer cells.
RPMI 1640 is distinctive from other media in that it contains the reducing agent glutathione and high concentrations of vitamins. It contains biotin, vitamin B₁₂, and PABA, which are absent from MEM or DMEM, and additionally features exceptionally high levels of inositol and choline. The medium contains no proteins, lipids, or growth factors, thus requiring supplementation with other additives, typically 10% fetal bovine serum (FBS). It utilizes a sodium bicarbonate buffering system (2.0 g/L) and requires a 5–10% CO₂ atmosphere to maintain physiological pH.
This product contains no antibiotics or antimycotics.
It contains 10 mM HEPES, 4500 mg/L D‑glucose, 292 mg/L L‑glutamine, 110 mg/L sodium pyruvate, and 1500 mg/L sodium bicarbonate. The pH ranges from 7.0 to 7.2.
Precautions
1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination.
2. To maintain optimal performance, avoid freeze‑thaw cycles.
3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.
规格
规格或纯度
BioReagent
英文名称
RPMI 1640 Medium (4.5 g/L D-Glucose, 1.5 g/L Sodium Bicarbonate)
危险品化学品经营许可证（带存储）