计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞学常规染色有巴氏 (Papanicolaou) 染色、Rakoff 染色、姬姆萨染色等,Papanicolaou 染色和 Rakoff 染色均可用于检测阴道上皮雌激素水平、判定雌激素水平。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
Rakoff 染色液由淡绿、伊红等组成,操作简单,经常用于临床阴道细胞学涂片快速检测。
自备材料
1、细胞刷、试管、载玻片
2、生理盐水
操作步骤(仅供参考)
1、用细胞刷沿阴道侧壁刷取黏膜鳞状上皮细胞,放入装有 1~2ml 生理盐水的试管内。
2、在试管内滴入 3~5 滴Rakoff染色液,并轻轻混匀。
3、取 1~2 滴混合液于载玻片上,制成细胞涂片。
4、待涂片干燥,中性树胶封片。
染色结果
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注意事项
1、细胞涂片时应注意使细胞均匀。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Conventional cytological staining methods include Papanicolaou staining, Rakoff staining, Giemsa staining and so on. Both Papanicolaou staining and Rakoff staining can be used to detect and evaluate the estrogen level of vaginal epithelial cells. This reagent is for research use only and shall not be applied for clinical diagnosis or other purposes.
Composed of Light Green, Eosin and other components, Rakoff Staining Solution features simple operation and is commonly adopted for rapid staining of clinical vaginal cytological smears.
Required Materials
1.Cell brush, test tube, glass slide
2.Normal saline
Operating Procedures (For reference only)
1.Scrape mucosal squamous epithelial cells from the lateral vaginal wall with a cell brush, then place the cells into a test tube containing 1~2 mL normal saline.
2.Add 3~5 drops of Rakoff Stain into the test tube and mix gently.
3.Pipette 1~2 drops of the mixture onto a glass slide to prepare a cell smear.
4.Allow the smear to air-dry, then mount with neutral balsam.
Staining Results
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Precautions
1.Ensure uniform cell distribution when preparing smears.
2.Use the reagent as soon as possible after opening to avoid compromised staining performance.
3.Wear a lab coat and disposable gloves during operation for personal safety and health protection.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R1526193 | |
| 分析证书 | R1526193 |
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