计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,无RNA酶,用于核酸电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品是 10 倍浓缩的 RNA 甘油凝胶上样缓冲液,专用于 RNA 甲醛变性凝胶电泳,能促使 RNA 样品沉入凝胶加样孔中。其主要成份为甘油,EDTA,溴酚蓝和二甲苯青等,经过 DEPC 处理,无 RNase。溴酚蓝和二甲苯青用作电泳时的指示剂,可指示电泳进程。
操作步骤(仅供参考):
1.在 RNase free 的微量离心管中,依次加入甲酰胺 10 μL,37%甲醛 3.5 μL,10× RNA 甘油凝胶上样缓冲液2 μL,RNA 样品 5 ug,DEPC 水补足至 20 μL;
2.混匀后,在 95℃水浴中孵育 2 min(或 55℃,15 min),再迅速将离心管静置冰上冷却 2 min;
3.冷却后,直接上样到甲醛变性凝胶加样孔内,电泳。
注意事项:
1.仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内;
2.为了您的安全和健康,请穿戴好个人防护装备和服装进行操作;
3.请使用无 RNase 的溶液、微量离心管及枪头等,以防止 RNA 降解。
This product is a 10× concentrated RNA glycerol gel loading buffer, specifically designed for RNA formaldehyde denaturing gel electrophoresis. It facilitates the settling of RNA samples into the gel wells. Its main components include glycerol, EDTA, bromophenol blue, and xylene cyanol. It has been treated with DEPC and is RNase-free. Bromophenol blue and xylene cyanol serve as tracking dyes during electrophoresis to indicate the progress of the run.
Procedure (for reference only):
1.In an RNase-free microcentrifuge tube, sequentially add 10 μL of formamide, 3.5 μL of 37% formaldehyde, 2 μL of 10× RNA glycerol gel loading buffer, and 5 μg of RNA sample. Make up the volume to 20 μL with DEPC-treated water.
2.After mixing, incubate in a 95°C water bath for 2 min (or at 55°C for 15 min), then quickly place the tube on ice to cool for 2 min.
3.After cooling, load directly into the wells of a formaldehyde denaturing gel and perform electrophoresis.
Precautions:
1.This product is for research use by professionals only. It must not be used for clinical diagnosis or treatment, for food or drugs, or stored in ordinary residential buildings.
2.For your safety and health, please wear appropriate personal protective equipment and clothing during operation.
3.Please use RNase-free solutions, microcentrifuge tubes, and pipette tips to prevent RNA degradation.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 RNase free 级别指南 → 查看 for NA electrophoresis 级别指南 →