计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ex(nm):568 Em(nm):591
制品说明
ROX Reference Dye一般用于ABI、Stratagene等公司的Real Time PCR 扩增仪上,用于调整PCR加样误差所引起的PCR管与管之间的差异。不同仪器所需ROX Reference Dye浓度不同。我公司提供ROX Reference Dye 储存液,浓度为25µmol/L,使用终浓度根据不同仪器为50nmol/L 或500nmol/L,用户可以根据仪器的推荐浓度添加。
不同仪器ROX推荐使用终浓度:
Instrument | ROX per 50µL PCR Reaction | Final Concentration |
ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus | 1.0 µL(0.6~1.0µL) | 500nmol/L(300~500nmol/L) |
ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett Rotor Gene 3000 | 0.1 µL*(0.06~0.1µL) | 50nmol/L(30~50nmol/L) |
从配制PCR反应的水溶液中减去ROX染料的体积
*为使每个反应精确加入0.1µL,推荐使用前将ROX用水稀释10倍,并使用1µL稀释后的溶液。
Ex(nm):568 Em(nm):591
Product description
ROX Reference Dye is generally used on Real Time PCR amplification machines of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. Different instruments require different concentrations of ROX Reference Dye. Our company provides ROX Reference Dye storage solution with a concentration of 25μmol/L, and the final concentration of use is 50nmol/L or 500nmol/L depending on the instrument. The user can add it according to the recommended concentration of the instrument.
The recommended final concentration of ROX for different instruments:
Instrument | ROX per 50µL PCR Reaction | Final Concentration |
ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus | 1.0 µL(0.6~1.0µL) | 500nmol/L(300~500nmol/L) |
ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett Rotor Gene 3000 | 0.1 µL*(0.06~0.1µL) | 50nmol/L(30~50nmol/L) |
Subtract the volume of ROX dye from the aqueous solution used to prepare the PCR reaction
*In order to accurately add 0.1μL to each reaction, it is recommended to dilute ROX 10 times with water before use, and use 1μL of the diluted solution.