计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Description
Relaxed pHOT-1 is suited to assaying the supercoiling activity of DNA gyrase. It is also useful for assaying alterations in DNA linking number for assessing intercalation. Relaxed pHOT-1 DNA is prepared using high purity topo I relaxation reactions, followed by inactivation and re-purification of the relaxed product. Aladdin staff then verify the relaxation status of each lot and validates that the substrate can be supercoiled by E. coli and S. aureus DNA gyrase.
The plasmid pHOT1 contains the high affinity topo I cleavage site (the hexadecameric sequence described by Westergaard and co-workers, see Bonven et al., 1985) that is derived from the tetrahymena ribosomal gene repeat. This construct is useful as a topo I cleavage substrate since the enzyme efficiently cleaves at the hexadecameric site in the absence of camptothecin. This allows the investigator to rigorously evaluate new potential topo I inhibitors because a single background cleavage is a built-in control; if the agent stimulates cleavage at this and/or any other sequence in the fragment that contains the hexadecameric site, prominent cutting is readily revealed. Typically, it is difficult to trap topo I cleavages on DNA; one must use more enzyme since cleavage complexes consume the enzyme stoichiometrically. For this reason, higher levels of enzyme are required for analysis of DNA cleavages. The fragment can be isolated from the polylinker using the sites shown below (protocols in Maniatis cloning book), end labeled and used as a cleavage substrate.
Shipping&storage
Relaxed pHOT-1 DNA is shipped at ambient temperature and should be stored a 4°C.
规格
英文名称
Relaxed pHOT-1 DNA
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）