计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
该磁珠适用于RNA片段快速分选和回收。磁珠经过磁性分离和乙醇清洗，低盐洗脱缓冲液或无核酸酶水洗脱的高纯度RNA片段不含有核苷酸、引物、酶和盐等污染物，可被直接用于下游应用，例如：测序、杂交、RT-PCR反应等，并可应用于手动或自动液体操作设备。
产品特点
1、回收率可达80%以上；
2、操作快速简单，20min即可完成整个操作流程，无需离心。
使用建议
1. RNA回收效率低或者没有？
1）RNA发生降解。操作过程要严格的确保无RNase的污染。
2）样本质量低。
3）洗脱效率低。80%乙醇需现用现配，放置时间过久，可能由于乙醇挥发，造成浓度降低，影响洗脱效果；
4）洗脱溶液孵育时间过短。磁珠应于洗脱液中按规定时间孵育，常规为5 min；
5）磁珠过度干燥。切勿室温干燥磁珠大于10 min，过度干燥将降低洗脱效率。
2. 纯化RNA中有磁珠残留？
1）磁珠分离时间过短或磁力器磁力较弱。增加分离时间或选用磁力强的磁力器，确保磁珠被磁力器全部吸附；
2）洗脱步骤中，吸取上清速度过快。缓慢吸取上清，注意切勿吸取磁珠。
3. 如何检测回收后RNA的纯度？
可以通过测定A260/A280的比值来评估。
保存条件
4℃保存。
操作流程
1、将 RNA 和 RNase Free Water 按 1：1.5 混合，将稀释后的 RNA 样品与 1.8 倍体积的磁珠混合，室温静置 5min；
2、通过磁力器进行分离，室温静置 5min，并吸除上清；
3、80%乙醇漂洗一次（此过程中样品管保持在磁力器上）；
4、再次用 80%乙醇漂洗一次（此过程中样品管保持在磁力器上）；
5、吸除上清，室温静置 5min，用适量 RNase Free Water 的水进行洗脱，室温静置 5min；
6、磁力器分离，室温静置 5min，转移上清至-20℃储存。
The magnetic beads are suitable for rapid sorting and recovery of RNA fragments. Magnetic beads are magnetically separated and washed with ethanol. The high-purity RNA fragments eluted with low-salt elution buffer or nuclease-free water do not contain contaminants such as nucleotides, primers, enzymes, and salts, and can be directly used in downstream applications. For example: sequencing, hybridization, RT-PCR reaction, etc., and can be applied to manual or automatic liquid handling equipment.
Features
1. The recovery rate can reach more than 80%;
2. The operation is fast and simple, and the entire operation process can be completed in 20 minutes without centrifugation.
Recommendations
1. Inefficient or no RNA recovery?
1) RNA is degraded. The operation process should be strictly ensured that there is no RNase contamination.
2) The sample quality is low.
3) The elution efficiency is low. 80% ethanol needs to be used and prepared immediately. If it is stored for a long time, the concentration may decrease due to the volatilization of ethanol, which will affect the elution effect;
4) The incubation time of the elution solution is too short. The magnetic beads should be incubated in the eluent for a specified time, usually 5 min;
5) Magnetic beads are over-dried. Do not dry the beads at room temperature for more than 10 minutes, as excessive drying will reduce the elution efficiency.
2. Are there magnetic beads remaining in the purified RNA?
1) The separation time of the magnetic beads is too short or the magnetic force of the magnetizer is weak. Increase the separation time or choose a magnetizer with strong magnetic force to ensure that the magnetic beads are all adsorbed by the magnetizer;
2) During the elution step, the supernatant was aspirated too fast. Aspirate the supernatant slowly, taking care not to aspirate the beads.
3. How to check the purity of recovered RNA?
It can be assessed by measuring the ratio of A260/A280.
Storage Conditions
Store at 4°C.
Operating procedures
1. Mix RNA and RNase Free Water at a ratio of 1:1.5, mix the diluted RNA sample with 1.8 times the volume of magnetic beads, and let stand at room temperature for 5 minutes;
2. Separate by a magnetic device, let stand at room temperature for 5 min, and remove the supernatant by suction;
3. Rinse once with 80% ethanol (the sample tube is kept on the magnet during this process);
4. Rinse once again with 80% ethanol (the sample tube remains on the magnet during this process);
5. Remove the supernatant by suction, let stand for 5 minutes at room temperature, elute with an appropriate amount of RNase Free Water, and let stand for 5 minutes at room temperature;
6. Magnetic separation, let stand for 5 min at room temperature, transfer the supernatant to -20 °C for storage.
规格
英文名称
RNA Clean Beads
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 B2302559 |
分析证书 | R427280 |
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）