计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无菌,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品是利用细胞内外存在盐离子浓度差而导致细胞膜胀破的原理来裂解无核红细胞的,主要成分为EDTA、NaHCO₃、NH₄Cl,该产品为1×浓度,可直接进行使用。
使用说明(仅供参考):
1、1倍体积的新鲜全血,加入3倍体积的1×红细胞裂解液。如1ml新鲜全血加入3ml红细胞裂解液,轻轻涡旋或颠倒混匀。
2、冰上放置15分钟,其间轻轻涡旋混匀两次,红细胞裂解后,溶液应该是清亮透明的。
3、收集细胞:4℃,450×g离心10分钟以沉淀白细胞,小心吸弃上清液。
4、向白细胞沉淀中加入两倍体积的红细胞裂解液,轻轻涡旋充分重悬白细胞。如起始血液为1ml,则加入2ml的红细胞裂解液。
5、4℃,450×g离心10分钟以沉淀白细胞,小心吸弃上清液。
6、重悬细胞,用于后续实验:如提取RNA,最好于此步开始使用DEPC水配制的溶液进行操作。(组织细胞处理:新鲜组织经胰酶或胶原酶等消化分散成单个细胞悬液,离心弃去上清液,其余同上。)
注意事项:
1、为了您的安全与健康,请穿实验服并戴一次性手套操作。
2、本品仅限用于哺乳动物类无核红细胞裂解(不适用于有核红细胞,如禽类等)。
This product lyses anucleated red blood cells based on the principle that the difference in salt ion concentration inside and outside cells causes the cell membrane to swell and rupture. Its main components are EDTA, NaHCO₃ and NH₄Cl. The product is at 1× concentration and can be used directly.
Instructions for Use (For Reference Only):
1. Add 3 volumes of 1× Red Blood Cell Lysis Buffer to 1 volume of fresh whole blood. For example, add 3 mL of lysis buffer to 1 mL of fresh whole blood, then mix gently by vortexing or inverting the tube.
2. Incubate on ice for 15 minutes, and gently vortex to mix twice during the incubation. The solution shall become clear and transparent after complete red blood cell lysis.
3. Cell collection: Centrifuge the sample at 450×g for 10 minutes at 4 °C to pellet leukocytes, then carefully aspirate and discard the supernatant.
4. Add 2 volumes of Red Blood Cell Lysis Buffer to the leukocyte pellet, and gently vortex to fully resuspend the leukocytes. For an initial blood volume of 1 mL, add 2 mL of the lysis buffer.
5. Centrifuge again at 450×g for 10 minutes at 4 °C to pellet leukocytes, then carefully aspirate and discard the supernatant.
6. Resuspend the cell pellet for subsequent experiments: If RNA extraction is required, it is recommended to use solutions prepared with DEPC-treated water starting from this step. (Tissue cell treatment: Digest fresh tissues with trypsin, collagenase or other digestive enzymes to prepare a single-cell suspension. Centrifuge and discard the supernatant, then follow the same procedures described above.)
Precautions:
1. For personal safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is only applicable for lysis of anucleated red blood cells of mammals; it is not suitable for nucleated red blood cells (e.g., avian red blood cells).
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R767015 | |
| 分析证书 | R767015 | |
| 分析证书 | R767015 | |
| 分析证书 | R767015 | |
| 分析证书 | R767015 | |
| 分析证书 | R767015 |
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