Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
规格
产品名称
AF532标记的链霉亲和素
别名
重组链霉亲和素 | 链霉亲和素
英文别名
SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin
规格或纯度
ExactAb™, 已验证, 1.0 mg/mL
生物活性
Binding Biotin
表达系统
E. coli
无载体
No
无动物源
No
偶联
AF532
Excitation(nm)
Green Laser(532nm)
Emission(nm)
555nm
Ex/Em(nm)
Ex:530nm;Em:555nm
来源
重组表达
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
ombinant Streptavidin Protein (AF532) (rp177478) – IF/ICC IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 4% PFA for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab190146) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (AF532) (rp177478) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (AF532) (rp177478) - Flow Cytometry Flow cytometry analysis of EpCAM expression by HT-29 cells (red) compared to an isotype control (blue). Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab190146) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF532) (rp177478) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
IF/ICC
1-10μg/mL
Flow Cytometry
1.0 -10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
ZJ25F0825097
危险品化学品经营许可证（带存储）