NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding. Features include: • Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin • Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin • No RYD recognition sequence—no known off-target binding domains like streptavidin • Affordable—significantly less expensive than streptavidin NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
Nuetral Avidin | NeutrAvidin | NeutrAvidin - PE Conjugated
规格或纯度
ExactAb™, 已验证, Ex:565nm, Em:575nm, 1.0 mg/mL
生物活性
Binding Biotin
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
来源
天然
预测分子量
60kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间，避免长时间暴露在光线下。切勿冷冻。
图片
NeutrAvidin Protein (PE) (rp188520) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with NeutrAvidin Protein (PE) (rp188520) at 0.3μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ24F1011587
危险品化学品经营许可证（带存储）