Doxycyclase is a water-soluble protein that uses a recombinant gene and appears as a colorless liquid. Recombinant gene protein based on the chemical structure of doxycycline. The active center of its gene fragment can destroy the chemical structural formula of doxycycline, so that the antimicrobial properties of the drug are lost after ring opening/chain breaking. When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly. Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane. During sterility test, take 2ml of enzyme and add 1500ml of washing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. A manual syringe was used to Pierce the respiratory mouth of the three incubators, and 1ml of enzyme was added to each of them, and the enzyme was spread on the entire surface of the filter membrane as far as possible. Then, the high concentration of enzyme was kept in full contact with the filter membrane of the incubator for 10 minutes, so as to destroy (neutralize and inactivate) the residual doxycycline drug on the filter membrane, and then pumped into the corresponding medium and shook well. Positive pairs were treated with 1ml of corresponding test bacteria. Adding 2ml of doxycyclase to the rinse solution can remove a small amount of drug antibacterial activity of doxycycline remaining in the membrane. Adding 1ml of doxycyclase to three incubators can remove a small amount of drug antibacterial activity of doxycycline remaining on the inner wall of the incubator and on the surface of the filter membrane. Shake the positive pair gently once a day in the morning and afternoon. Customers can do methodological verification according to the above, but also according to the actual operation of the verification.
规格
产品名称
盐酸多西环素酶
别名
盐酸多西环素酶
英文别名
Doxycycline hydrochloride enzyme
规格或纯度
EnzymoPure™, BioReagent, 无菌
分子类型
酶
产品线
生物产品
储存与运输
物理形态
液体
储存条件
2-8°C储存
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Upon receipt, it is recommended to aliquot.
ZJ25F0420209
危险品化学品经营许可证（带存储）