基本描述

产品名称	盐酸多西环素酶
别名	盐酸多西环素酶
英文别名	Doxycycline hydrochloride enzyme
规格或纯度	EnzymoPure™, BioReagent, 无菌
产品介绍	多西环素酶是采用重组基因的水溶性蛋白，呈无色液体。根据多西环素的化学结构而构建的重组基因蛋白。其基因片断的活性中心，可破坏多西环素的化学结构式，使开环/断链后失去该药物的抗菌性。做抗生素的无菌检查时，应用手工注射器吸取无菌水注入药物的西林瓶中，摇匀，溶解，再吸出。加入至0.9%氯化钠的500ml溶液中，摇匀，不可用培养器上的针头溶解，输入，避免高浓度的溶液通过滤膜，造成难以冲洗彻底。这里，再次强调：做抗生素的无菌检查时，一定要手工注射器注入无菌水溶解样品，再把溶解的样品转移至0.9%氯化钠的500ml溶液中摇匀，使得样品不会造成局部浓度过高，通过滤膜时难以冲洗彻底的情况。无菌检验时，取酶2ml放入1500ml的冲洗液摇匀。待冲洗液冲洗培养器的滤膜完成后，泵尽。用手工注射器在三个培养器的呼吸口刺入，各加1ml的酶，尽量让酶平铺在滤膜整个面，静止10分钟，使高浓度的酶与培养器滤膜充分接触，以破坏（中和、灭活）滤膜上残留的多西环素药物，然后再泵入相应的培养基，摇匀。阳性对照管加入1ml的对应试验菌。在冲洗液加2ml的多西环素酶，可以去除滤膜内部残留的多西环素的少量药物抗菌性。在三个培养器加1ml的多西环素酶，可以去除培养器内部壁上和滤膜表面残留多西环素的少量药物抗菌性。每天上午、下午各对阳性对照管轻轻摇匀一次。客户可根据上述做方法学验证，也可根据实际操作情况做验证。 Doxycyclase is a water-soluble protein that uses a recombinant gene and appears as a colorless liquid. Recombinant gene protein based on the chemical structure of doxycycline. The active center of its gene fragment can destroy the chemical structural formula of doxycycline, so that the antimicrobial properties of the drug are lost after ring opening/chain breaking. When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly. Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane. During sterility test, take 2ml of enzyme and add 1500ml of washing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. A manual syringe was used to Pierce the respiratory mouth of the three incubators, and 1ml of enzyme was added to each of them, and the enzyme was spread on the entire surface of the filter membrane as far as possible. Then, the high concentration of enzyme was kept in full contact with the filter membrane of the incubator for 10 minutes, so as to destroy (neutralize and inactivate) the residual doxycycline drug on the filter membrane, and then pumped into the corresponding medium and shook well. Positive pairs were treated with 1ml of corresponding test bacteria. Adding 2ml of doxycyclase to the rinse solution can remove a small amount of drug antibacterial activity of doxycycline remaining in the membrane. Adding 1ml of doxycyclase to three incubators can remove a small amount of drug antibacterial activity of doxycycline remaining on the inner wall of the incubator and on the surface of the filter membrane. Shake the positive pair gently once a day in the morning and afternoon. Customers can do methodological verification according to the above, but also according to the actual operation of the verification.

AI解读

产品规格参数

物理形态	液体
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	Store at 2-8℃ long term (12 months). Upon receipt, it is recommended to aliquot.
分子类型	酶

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批号(Lot Number)	证书类型	日期	货号
ZJ25F0420209	分析证书	25-04-09	rp216619

溶液计算器

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复溶计算器

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盐酸多西环素酶

库存信息