NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding. Features include: • Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin • Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin • No RYD recognition sequence—no known off-target binding domains like streptavidin • Affordable—significantly less expensive than streptavidin NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
规格
产品名称
Oregon Green 488标记的中性亲和素
别名
中性亲和素蛋白 | NeutrAvidin 蛋白 | 亲和素
英文别名
Nuetral Avidin | NeutrAvidin
规格或纯度
ExactAb™, 已验证, 1.0 mg/mL
生物活性
Binding Biotin
偶联
Oregon Green 488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
526nm
Ex/Em(nm)
Ex:498nm;Em:526nm
来源
天然
预测分子量
60kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
NeutrAvidin Protein (Oregon Green 488) (rp217318) - IF IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with NeutrAvidin Protein (Oregon Green 488) (rp217318) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
NeutrAvidin Protein (Oregon Green 488) (rp217318) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with NeutrAvidin Protein (Oregon Green 488) (rp217318) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
0.5-10μg/mL
IF/ICC
1-10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
ZJ26F0130887
危险品化学品经营许可证（带存储）