Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
规格
产品名称
LumiDye™ Blue 675标记的链霉亲和素
别名
重组链霉亲和素 | 链霉亲和素
英文别名
SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
0.2 mg/mL
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant Streptavidin Protein (LumiDye™ Blue 675) (rp220571) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (LumiDye™ Blue 675) (rp220571) at 0.5μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
2-20μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.