Pancreatic lipase when prepared essentially free of colipase is strongly inhibited by conjugated bile salts at or above their critical micell concentration. Addition of colipase restores activity to the bile-salt-inhibited lipase and gel filtration experiments indicate that colipase in bile salt solution makes a dimer which forms a 1:1 complex with lipase. 【Properties】 Lipase and colipase interact as a stoichiometrical relationship and classified colipase interact as a co-enzyme for lipase. The colipase is a single chain polypeptide with a molecular weight approximately 11,000 with 5 disulfide bridges and isoelectric point 5.0. 【Application】 Use Colipase as a co-emulslfier in diagnostic tests for the determination of lipase activity in combination with chromogenic Lipase Substrate. 【Attention】 For your safety and health, please wear Lab coat and disposable gloves.
Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.
ZJ26F0838483
危险品化学品经营许可证（带存储）