Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Streptavidin-R-PE conjugate comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms. This Streptavidin-R-PE product has been tested by immunofluorescent staining with flow cytometric analysis and Multiplex bead-based assay.
规格
产品名称
PE标记的链霉亲和素
别名
重组链霉亲和素 | 链霉亲和素 | R-藻红蛋白偶联物 | 链霉素亲和素 R PE | 重组Streptavidin蛋白(PE)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
来源
重组表达
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 0.05% Proclin300.
防腐剂
0.05% Proclin300
浓度
1.0 mg/mL
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant Streptavidin Protein (PE) (rp230629) - Multiplex bead-based assay Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected. ·Capture Antibody (Mouse IgG1): 3 μg/scale ·
Detection Antibody (Biotin goat Anti-Mouse IgG1): 2500 ng/mL ·
SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000 Recombinant Streptavidin Protein (PE) (rp230629) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis. Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.
Recombinant Streptavidin Protein (PE) (rp230629) - Multiplex bead-based assay Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected. ·Capture Antibody (Mouse IgG1): 3 μg/scale ·Detection Antibody (Biotin goat Anti-Mouse IgG1): 10 ng/mL ·SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000 Recombinant Streptavidin Protein (PE) (rp230629) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis. Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.
Recombinant Streptavidin Protein (PE) (rp230629) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.5μg/mL for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (PE) (rp230629) at 0.3μg/mL for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
0.1-10μg/mL Multiplex bead-based assay: Assay-Dependent Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
危险品化学品经营许可证（带存储）