Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Streptavidin-R-PE conjugate comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms. This Streptavidin-R-PE product has been tested by immunofluorescent staining with flow cytometric analysis and Multiplex bead-based assay.
规格
产品名称
PE标记的链霉亲和素
别名
重组链霉亲和素 | 链霉亲和素 | R-藻红蛋白偶联物 | 链霉素亲和素 R PE | 重组Streptavidin蛋白(PE)
Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.
生物活性
Binding Biotin
表达系统
E. coli
无载体
Yes
无动物源
Yes
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
来源
重组表达
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 0.01% Thimerosal.
防腐剂
0.01% Thimerosal
浓度
1.0 mg/mL
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant Streptavidin Protein (PE) (rp230630) - Multiplex bead-based assay Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected. ·Capture Antibody (Mouse IgG1): 3 μg/scale ·Detection Antibody (Biotin goat Anti-Mouse IgG1): 2500 ng/mL ·SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000 Recombinant Streptavidin Protein (PE) (rp230630) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis. Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.
Recombinant Streptavidin Protein (PE) (rp230630) - Multiplex bead-based assay Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected. ·Capture Antibody (Mouse IgG1): 3 μg/scale ·Detection Antibody (Biotin goat Anti-Mouse IgG1): 10 ng/mL ·SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000 Recombinant Streptavidin Protein (PE) (rp230630) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis. Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.
Recombinant Streptavidin Protein (PE) (rp230630) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.5μg/mL for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (PE) (rp230630) at 0.3μg/mL for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
0.1-10μg/mL Multiplex bead-based assay: Assay-Dependent Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
危险品化学品经营许可证（带存储）