阿拉丁提供重组蛋白A与LumiDye™ AF染料（表1）的偶联物。
表1 重组蛋白A偶联物的光谱性质
| Cat.No. | Conjugate | λmax (nm) | Em (nm |
| rp303252 | FITC | 498 | 517 |
| rp303253 | AF488 | 490 | 525 |
| rp303254 | AF555 | 553 | 568 |
| rp303256 | AF594 | 590 | 618 |
| rp303258 | AF647 | 650 | 668 |
阿拉丁重组蛋白A是基于天然金黄色葡萄球菌蛋白A进行结构改造的蛋白。它由四个Fc结合结构域片段连接而成，分子量为27KD。与天然蛋白A相比，它去除了与免疫球蛋白Fab区域的结合位点，增强了与Fc区域的结合能力，并提高了蛋白的稳定性和耐碱性。重组蛋白A可用于从腹水、血清、细胞培养上清液或细胞提取物中分离和纯化不同哺乳动物亚型的抗体或含有抗体Fc片段的基因工程重组蛋白。
重组蛋白A在最佳条件下与LumiDye™ AF555进行偶联，并经纯化去除未结合的游离染料。该偶联物可用于流式细胞术和免疫荧光染色中的免疫球蛋白检测，以及检测方法的开发。橙色荧光探针最大吸收/发射波长约为553nm和568 nm。
应用
流式细胞术：1:100-1:1000
免疫荧光/免疫细胞化学：1:500
注意：由于染色方案因应用而异，蛋白A偶联物的适当稀释度应通过实验经验确定。
Introduction
Aladdin offers recombinant protein A conjugated to our LumiDye™ AF Dyes (Table 1).
Table 1. Spectral characteristics of protein A conjugates.
| Cat.No. | Conjugate | λmax (nm) | Em (nm |
| rp303252 | FITC | 498 | 517 |
| rp303253 | AF488 | 490 | 525 |
| rp303254 | AF555 | 553 | 568 |
| rp303256 | AF594 | 590 | 618 |
| rp303258 | AF647 | 650 | 668 |
Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts.
Recombinant Protein A was conjugated with LumiDye™ AF555 under optimal conditions and subsequently purified to remove unconjugated free dyes. This conjugate can be used for immunoglobulin detection in Flow Cytometry and IF and for assay development. Orange Fluorescence. Excitation/Emission wavelength= 553 nm/568 nm.
Application
Flow Cytometry: 1:100-1:1000
IF/ICC: 1:500
Note: Because staining protocols vary with application, the appropriate dilution of the protein A conjugates should be determined empirically.
ZJ26F0434995
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