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224,000+ 科研产品 · 三重ISO认证 · 每个产品均提供COA和SDS · 现货产品当日发货

AF647标记的重组蛋白A/G

货号: rp303278
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Accession #
P19909,P02976
蛋白标签
No tag
表达系统
E. coli
★
规格
库存
价格
数量
100μg
rp303278-100μg
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500μg
rp303278-500μg
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¥1,899.90
1mg
rp303278-1mg
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¥2,999.90
5mg
rp303278-5mg
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AF647标记的重组蛋白A/G
概述 规格 储存与运输 图片 技术文档 高级数据 质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器 常见问题
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为什么选择此级别

BioReagent,无叠氮钠,适用于免疫荧光(IF) 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。

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2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

  阿拉丁提供重组蛋白A/G与LumiDye™ AF染料的偶联物（表1）。

  表1. 蛋白A/G偶联物的光谱特性

Cat.No.Conjugateλmax (nm)Em (nm
rp303272HRPNANA
rp303273FITC498517
rp303275AF488490525
rp303276AF555553568
rp303277AF594590618
rp303278AF647650668
rp302416Cy3554566

   阿拉丁重组蛋白A/G是一种基因工程蛋白，包含5个蛋白A的IgG结合区和2个蛋白G的IgG结合区。该重组A/G-Cys蛋白已去除了细胞壁结合区、细胞膜结合区和白蛋白结合区，以确保最大程度的特异性IgG结合。该重组蛋白A/G是制备用于纯化多克隆或单克隆IgG抗体的亲和凝胶的理想选择。蛋白A/G能结合多种人、小鼠和大鼠的IgG亚类（例如：人IgG1, IgG2, IgG3, IgG4；小鼠IgG2a, IgG2b, IgG3；大鼠IgG2a, IgG2c）。它还能结合牛、山羊、绵羊、马、兔、豚鼠、猪、狗和猫的总IgG。

   我们检测了不同IgG亚型与重组蛋白A/G的结合数据，如图所示（图1）。

图1. 蛋白A/G对选定物种的亲和力

  重组蛋白A/G在最佳条件下与进行偶联 LumiDye™ AF647，并经纯化去除未结合的游离染料。该偶联物可用于流式细胞术和免疫荧光染色中的免疫球蛋白检测，以及检测方法的开发。红色荧光探针最大吸收/发射波长约为650nm和668nm。

应用：

免疫荧光/免疫细胞化学：1:500

注意： 由于染色方案因应用而异，蛋白A/G偶联物的适当稀释度应通过实验确定。

  Aladdin offers recombinant protein A/G conjugated to our LumiDye™ AF Dyes (Table 1).

Table 1. Spectral characteristics of protein A/G conjugates.

Cat.No.Conjugateλmax (nm)Em (nm
rp303272HRPNANA
rp303273FITC498517
rp303275AF488490525
rp303276AF555553568
rp303277AF594590618
rp303278AF647650668
rp302416Cy3554566

 

Aladdin recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant A/G-Cys to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for making affinity gel to purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

We have detected the binding data of different IgG subtypes with recombinant protein A/G, as shown in the Graph1.

Graph 1. Protein A/G affinity to selected species.

  Recombinant Protein A/G was conjugated with LumiDye™ AF647 under optimal conditions and subsequently purified to remove unconjugated free dyes. This conjugate can be used for immunoglobulin detection in Flow Cytometry and IF and for assay development. Red Fluorescence. Excitation/Emission wavelength= 650 nm/668 nm.

Application：

IF/ICC: 1:500

Note: Because staining protocols vary with application, the appropriate dilution of the protein A conjugates should be determined empirically.

规格

产品名称
AF647标记的重组蛋白A/G
别名
AF647标记的重组蛋白A/G | 重组蛋白A/G-AF647
英文别名
Protein A/G- AF647 | Recombinant Protein A/G- AF647 | AF647-Recombinant Protein A/G | Recombinant protein A/G, AF647 conjugated | reProtein A/G- AF647
规格或纯度
无叠氮钠,BioReagent,适用于免疫荧光(IF),1.0 mg/mL
生化机理
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A.
表达系统
E. coli
种属
金黄色葡萄球菌(Staphylococcus aureus)
氨基酸
35-330 aa & 373-497 aa
序列
NAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDSLEGSGSGTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE
蛋白标签
No tag
无载体
No
无动物源
No
偶联
AF647
Excitation(nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
来源
重组表达
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
浓度
1.0 mg/mL
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.
图片
Recombinant Protein A/G, AF647 conjugate (rp303278) – IF/ICC 
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902; Isotype: Human IgG1) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Protein A/G, AF647 conjugate (rp303278) at 1/500 dilution for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at 1/1000 dilution for 5 minutes. Images were taken on the confocal laser scanning microscope. .
Recombinant Protein A/G, AF647 conjugate (rp303278) – IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Catumaxomab (anti-CD3&EpCAM) (Ab175489; Isotype: Mouse IgG2a & Rat IgG2b) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Protein A/G, AF647 conjugate (rp303278) at 1/500 dilution for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at 1/1000 dilution for 5 minutes. Images were taken on the confocal laser scanning microscope.
Recombinant Protein A/G, AF647 conjugate (rp303278) – IF/ICC 
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839; Isotype: Rabbit IgG) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Protein A/G, AF647 conjugate (rp303278) at 1/500 dilution for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at 1/1000 dilution for 5 minutes. Images were taken on the confocal laser scanning microscope.

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批号(Lot Number) 证书类型 货号
ZJ26F0434919 分析证书 rp303278
ZJ26F0434918 分析证书 rp303278
ZJ26F0434917 分析证书 rp303278
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