Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
规格
产品名称
AF546标记的链霉亲和素
别名
重组链霉亲和素 | 链霉亲和素
英文别名
SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
图片
Recombinant Streptavidin Protein (AF546) (rp639012) - IF IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (AF546) (rp639012) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (AF546) (rp639012) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF546) (rp639012) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0622389
危险品化学品经营许可证（带存储）