蔗糖-PBS溶液(Sucrose-PBS Solution)，是一种含5%、10%、20%或30%浓度蔗糖的PBS溶液，本产品适用于免疫组化(IHC)、免疫荧光(IF)、免疫细胞化学(ICC)和流式分析(Flow Cytometry)等组织、细胞等生物样品的脱水，也可作为特殊样本的脱水剂和冷冻保护剂。使用蔗糖-PBS溶液进行梯度脱水，可以有效防止冰冻切片时样本内产生冰晶而影响后续的切片和检测。
PBS (Phosphate-Buffered Saline)是磷酸缓冲盐溶液的简称，主要由NaCl、KCl、Na2HPO4和NaH2PO4组成。PBS具有缓冲能力，能够维持渗透压平衡，保持细胞组织形态，在细胞培养、固定等过程中可用于细胞的洗涤等；蔗糖具有脱水作用，常用作脱水剂和冷冻保护剂。
本产品为即用型，使用便捷，无须配制或稀释。
操作步骤：
1.新鲜组织取材或经灌注组织取材，先置于5-20倍体积的固定液中进行固定，4ºC固定8-24h (视组织块大小而定)。
2.先置于5-20倍体积的5%蔗糖-PBS溶液中，4ºC脱水12-24h(时间视组织块大小而定)，当组织块完全沉底时，即可置换下一梯度脱水剂，即后续使用10%蔗糖-PBS溶液、20%蔗糖-PBS溶液和30%蔗糖-PBS溶液依次进行脱水处理。脱水处理的条件和5%蔗糖-PBS溶液一致。脱水处理完成后，若暂时不进行切片，可置于30%蔗糖-PBS溶液中于4℃冰箱保存较长时间。
3.后续根据实验要求进行包埋等操作。
注意事项：
1.蔗糖-PBS溶液经过滤除菌处理，使用过程中应尽量避免细菌污染。
2.组织块脱水前先用免疫染色固定液充分固定，较大块组织需修整成小块再固定（1.0×1.0×0.2cm）。
3.组织块放入脱水剂中脱水，脱水剂是组织块体积的 5-20倍，以达到组织充分覆盖均匀脱水的效果。
4.组织脱水时间长短取决于组织大小，通常以组织快沉底为宜。
5.若样本在30%蔗糖-PBS溶液中较长时间保存，须定期观察组织情况，尽快进行后续处理，防止组织霉变损坏。
6.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
7.为了您的安全和健康，请穿实验服并戴一次性手套操作。
Sucrose-PBS Solution refers to PBS solutions containing sucrose at concentrations of 5%, 10%, 20% or 30%. This product is suitable for dehydration of biological samples such as tissues and cells in immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), flow cytometry and other assays. It can also serve as a dehydrating agent and cryoprotectant for special specimens. Gradient dehydration with Sucrose-PBS Solution effectively prevents ice crystal formation inside samples during frozen sectioning, which would otherwise compromise subsequent sectioning and detection procedures.
PBS, short for Phosphate-Buffered Saline, is mainly composed of NaCl, KCl, Na₂HPO₄ and NaH₂PO₄. Featuring buffering capacity, PBS maintains osmotic balance and preserves the morphological integrity of cells and tissues; it is widely used for cell washing in cell culture, fixation and other experimental workflows. Sucrose exerts a dehydrating effect and is commonly utilized as a dehydrating agent and cryoprotectant.
This product is ready-to-use for convenient application, with no need for preparation or dilution.
Operating Procedures:
1. Collect fresh tissues or perfused tissues, then immerse them in fixative solution at a volume 5–20 times that of the tissue blocks for fixation at 4°C for 8–24 hours (duration adjusted according to tissue block size).
2. Transfer tissues to 5% Sucrose-PBS Solution (5–20 times the tissue volume) for dehydration at 4°C over 12–24 hours, with incubation time determined by tissue dimensions. Once tissue blocks sink completely to the bottom of the container, replace the solution with the next gradient dehydrating agent: sequentially conduct dehydration in 10%, 20% and 30% Sucrose-PBS Solution under identical incubation conditions as the 5% formulation. If sectioning is not performed immediately after full dehydration, tissues may be stored long-term in 30% Sucrose-PBS Solution in a refrigerator at 4°C.
3. Proceed with embedding and other downstream procedures as required by the experimental protocol.
Precautions:
1. Sucrose-PBS Solution has been filter-sterilized. Avoid bacterial contamination during handling.
2. Fully fix tissue blocks with immunostaining fixative prior to dehydration. Trim large tissues into small pieces (1.0 × 1.0 × 0.2 cm) before fixation.
3. Immerse tissue blocks in dehydrating agent at a volume ratio of 5–20:1 relative to the tissue to ensure full coverage and uniform dehydration.
4. Dehydration duration varies with tissue size; complete dehydration is confirmed when tissues sink rapidly to the bottom of the solution.
5. For specimens stored long-term in 30% Sucrose-PBS Solution, regularly inspect tissue conditions and complete subsequent processing promptly to prevent mildew and tissue deterioration.
6. This product is restricted to scientific research by professional personnel only. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical manufacturing, nor stored in residential premises.
7. Wear a lab coat and disposable nitrile gloves throughout the operation for personal safety and health protection.
ZJ26F0131147
危险品化学品经营许可证（带存储）